C4A

Host Animal Mouse
Specie Reactivity Human
Antibody Clone CTJS-417
Isotype IgG1κ
Applications WB; ELISA
Host Animal Rabbit
Specie Reactivity Human
Antibody Clone CTJS-169
Isotype IgG
Applications WB; IF; IHC
Host Animal Rabbit
Specie Reactivity Human
Isotype IgG
Applications WB; IHC
Host Animal Rabbits
Isotype IgG
Applications Western Blots; ELISA; Immunodiffusion
Clear All
Specie Reactivity Human
Sensitivity 0.94 ng/mL (1.56-100 ng/mL)
Applications ELISA
Specie Reactivity Human
Sensitivity 0.152 ng/mL (0.312-20 ng/mL)
Applications ELISA
Specie Reactivity Human
Sensitivity 7.81 ng/mL (31.25-2000 ng/mL)
Applications ELISA
Specie Reactivity Human
Sensitivity 0.76 ng/mL (0.78-200 ng/mL)
Applications ELISA
Specie Reactivity Mouse
Sensitivity 13.3 pg/mL (31.2-2000 pg/mL)
Applications ELISA
Specie Reactivity Mouse
Sensitivity 9.75 pg/mL (39-2500 pg/mL)
Applications ELISA
Specie Reactivity Rat
Sensitivity 0.145 ng/mL (0.312-20n g/mL)
Applications ELISA
Specie Reactivity Rat
Sensitivity 15.6 ng/mL (62.5-4000 ng/mL)
Applications ELISA
Specie Reactivity Rabbit
Sensitivity 1.875 ng/mL (3.125-200 ng/mL)
Applications ELISA
Specie Reactivity Chicken
Sensitivity 1.875 ng/mL (3.125-200 ng/mL)
Applications ELISA
Specie Reactivity Ovine
Sensitivity 0.469 ng/mL (0.781-50 ng/mL)
Applications ELISA
Specie Reactivity Ovine
Sensitivity 0.1 ng/mL (5.0-100 ng/mL)
Applications ELISA
Specie Reactivity Porcine
Sensitivity 0.469 ng/mL (0.781-50 ng/mL)
Applications ELISA
Specie Reactivity Guinea Porcine
Sensitivity 0.375 ng/mL (0.625-40 ng/mL)
Applications ELISA
Specie Reactivity Guinea Porcine
Sensitivity 1.0 pg/mL (250-5000 pg/mL)
Applications ELISA
Specie Reactivity Bovine
Sensitivity 1.0 ng/mL (25-500 ng/mL)
Applications ELISA
Specie Reactivity Canine
Sensitivity 1.0 pg/mL (250-5000 pg/mL)
Applications ELISA
Clear All
Specie Reactivity Human
Type Recombinant Protein
Expression Host Wheat Germ (in vitro)
Applications WB; ELISA; PA; AP
Specie Reactivity Human
Type Recombinant Protein Antigen
Expression Host E. Coli
Applications AC
Specie Reactivity Human
Type Recombinant Protein Antigen
Expression Host E. Coli
Applications AC
Specie Reactivity Human
Type Recombinant Protein Antigen
Expression Host E. Coli
Applications AC
Specie Reactivity Human
Type Native Protein
Applications Functional Assays
Specie Reactivity Human
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA; IP
Specie Reactivity Human
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA; IP
Specie Reactivity Rat
Type Recombinant Protein
Expression Host Yeast
Applications WB; ELISA
Specie Reactivity Rat
Type Recombinant Protein
Expression Host E.coli/Mammalian cell/Baculovirus
Applications WB; ELISA
Specie Reactivity Human; Mouse; Rat
Purity >90%
Applications Blocking; WB
Specie Reactivity Human
Purity >90%
Applications Immunocompetition; Immunodepletion
Specie Reactivity Human; Mouse; Rat
Purity >85%
Applications Immunogen
[Cat#: CTApt-723] Anti-C4A Aptamer SELEX Service
Affinity (Kd) 1nM~1μM
Chemical Modification N/A
Clear All
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-AC-GFP
Tag GFP
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-AC-GFP
Tag GFP
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-Entry
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6 series
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-AC-GFP
Tag GFP
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-Entry
Specie Reactivity Rat
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Rat
Type Expression Plasmid
Vector pCMV6-Entry
Product List Background

Background

Human C4a is a cationic polypeptide that can be directly isolated from fresh serum after the activation of the complement cascade. The C4a anaphylatoxin consists of 77 residues without histidine, tryptophan, and carbohydrate side chains, weighing 8,759 Da. C4a is homologous to C3a and C5a (two anaphylatoxins released by complement activation) in sequence and structure within or between species. During the activation of the complement classical pathway and the lectin pathway, the alpha-chain of the complement component 4 (C4) is respectively cleaved by activated C1s and MASP-2, releasing a small fragment C4a and a large fragment C4b. The C4b is involved in the next step of the complement pathways in the formation of convertases. C4a serves as an anaphylatoxin implicated in inflammatory reactions.

Although similar in structure to other anaphylatoxins, the functional properties of C4a are different from C3a and C5a. Firstly, C4a has no specific receptor and seems to function through the C3a receptor. Secondly, C4a is an anaphylatoxin local inflammation, induces smooth muscle contraction, increases vascular permeability, and causes the release of histamine by mast cells and basophilic leukocytes, but these induced bio-functions are much weaker compared to C3a and C5a. Besides, C4a, as well as C3a, exhibit antibacterial activity against both Gram-negative and Gram-positive bacteria, which is independent of the receptor but depends on the net charge, the proportion of hydrophobic amino acids, and the amphiphilic peptide. It is indicated that the deficiency of the C4a might result in systemic lupus erythematosus and type I diabetes mellitus, and excess was associated with schizophrenia and psychotic bipolar disorder.

Fig. 1 Structure of C4a. (From Wikipedia: By Emw - Own work, https://commons.wikimedia.org/wiki/File:Protein_C4A_PDB_1hzf.png)Fig. 1 Structure of C4a.1

Reference

  1. From Wikipedia: By Emw - Own work, CC BY-SA 3.0 https://commons.wikimedia.org/wiki/File:Protein_C4A_PDB_1hzf.png
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For Research Use Only.