MRC-5-based Cytotoxicity Safety Screening Service

Precision Immunotherapy Starts with MRC-5 Insight

Creative Biolabs provides In Vitro ADME service aimed at supporting the development of immunotherapy. This service evaluates the absorption, distribution, metabolism, and excretion properties of candidate drugs using in vitro models. It ensures a thorough assessment of their safety and efficacy profiles in preclinical stages, crucial for advancing immunotherapy research.

We are proud to introduce our In Vitro Phenotyping Assay Services, including specialized MRC-5-based cytotoxicity safety screening service, tailored to bolster the development of immunotherapy treatments.

Our assay utilizes Beetle luciferin + ATP as the substrate, with tamoxifen serving as the control inhibitor. It is a functional assay conducted using MRC-5 cells, where cytotoxicity is assessed through luminescence detection, measuring the fluorescence response to evaluate cell viability and drug toxicity. This assessment ensures comprehensive safety and efficacy profiling in the preclinical stages of immunotherapy development.

Assay Information:

Why We Choose MRC-5?

• Representing human lung tissue, MRC-5 cells provide a biologically relevant model for assessing drug-induced cytotoxicity.
• Extensively validated for consistent performance in toxicity testing, ensuring robust and reproducible results.
• Widely recognized in biomedical research for their ability to mimic human tissue responses.

Workflow

• Cell Culture Preparation
  • MRC-5 cells are cultured in suitable growth media under controlled conditions.
• Assay Plate Preparation
  • 96-well assay plates are treated to enhance cell adhesion and stability.
• Cell Seeding and Incubation
  • MRC-5 cells are seeded into the assay plates at a specified density.
• Assay Setup
  • Test compounds, including tamoxifen as a control inhibitor, are prepared in various concentrations.
  • Beetle luciferin + ATP substrate mixture is added to each well-containing cells and test compounds.
• Incubation and Reaction Initiation
• The assay plates are incubated to allow for cellular uptake and metabolism of the substrate.
• Luminescence Detection
  • After the incubation period, luminescence is measured using a luminometer.
• Data Analysis
  • Fluorescence responses are quantified and analyzed to determine the cytotoxicity profiles of the tested compounds.

Other Services

Besides the above, we also offer the following services to enhance the precision and effectiveness of immunotherapy research, paving the way for innovative therapeutic solutions.

Applications

• Assessing the safety profiles of pharmaceutical compounds early in the development process, ensuring compliance with regulatory standards.
• Investigating cytotoxic effects induced by chemicals or biologics, leveraging the physiological relevance of MRC-5 cells.
• Supporting the evaluation of novel therapeutic candidates and formulations to optimize efficacy and safety parameters.

Advantages

• Utilizing MRC-5 cells in toxicity testing.
• Assessing cytotoxicity through luminescence detection of Beetle luciferin + ATP substrate, providing sensitive and quantitative results.
• Conducting functional assays under controlled conditions to ensure the reproducibility and reliability of data.

Our service rigorously evaluates the absorption, distribution, metabolism, and excretion properties of candidate drugs using advanced in vitro models, including MRC-5 cells. For researchers and pharmaceutical developers in need of precise cytotoxicity safety screening using MRC-5 cells, Creative Biolabs stands ready to assist, contact us today to discuss how we can accelerate your research and development goals.

For Research Use Only | Not For Clinical Use