In order to conduct complement system research more efficiently, Creative Biolabs has developed and established a variety of protocols to provide better products and services to our customers. Complement component C4 can be covalently bound to target surfaces in the activated state. When complement component C4 is cleaved by upstream components of the complement cascade, it forms two fragments, C4a and C4b. In this case, C4 fragments, C4a and C4b, can be obtained by incubating purified C4 with activated C1. Here, we describe the C4 fragments generation protocol for our clients all over the world.
C4 Fragments Generation
Fig.1 Flow chart of C4 fragments generation. (Creative Biolabs)
Published Data
Fig.2 Copy number variations of C4 and HERV-K and their association with systemic sclerosis susceptibility.1
Complement C4 copy number variants, implicated in autoimmune disorders, were examined for their role in systemic sclerosis risk. In a study of a number of subjects with validation, totals for C4, C4A, C4B, and HERV-K copy numbers were analyzed. Regression models stratified by C4A levels illustrated an interaction between C4A and C4B. Elevated total C4 copy number provided protection, whereas an imbalance between C4A and C4B heightened susceptibility, with differential protective effects by sex: men benefited from higher C4A, and women from increased C4B. Concordance between copy numbers, gene expression, and serum proteins underscores C4 genetics’ contribution to the major histocompatibility complex signal.
Based on these proven protocols and technologies, scientists at Creative Biolabs have completed many challenging projects in the complement system. We are therefore confident in offering the best products and services. If you do not find what you are interested in on the website, please feel free to contact us for a customized solution.
To learn more details, you can get access with the following links:
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Complement Products
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Complement Test Services
If you are interested in our products and services, please do not hesitate to contact us for more information.
Please note that our protocols are only for your reference!
Reference
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Kerick, Martin, et al. "Complement component C4 structural variation and quantitative traits contribute to sex-biased vulnerability in systemic sclerosis." NPJ Genomic Medicine 7.1 (2022): 57. Distributed under Open Access license CC BY 4.0, without modification.
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