Derived from the human leukocyte antigen (HLA) system, complement component 4 (C4) is an important protein in the complement system that, together with other components, performs many key functions in immunity, tolerance and autoimmunity. In order to better carry out research on the complement system, Creative Biolabs has developed many methods for the purification of various components. Here, we describe the C4 purification protocol for our clients all over the world.
C4 Purification
Fig.1 Flow chart of C4 purification protocol. (Creative Biolabs)
Published Data
For C4 protein isolation, the monoclonal antibody affinity column has been developed to distinguish C4-A from C4-B. Serum containing both isoforms was applied to an L003-coupled Sepharose column, then washed and eluted via a buffered pH gradient. Two clear peaks arose; SDS-PAGE analysis, based on the slower migration of the C4-A α-chain, confirmed their identities. Immunotyping with anti-Rodgers and anti-Chido sera verified the separation, with only minimal cross-contamination. Processing serum from individuals expressing a single allele yielded pure proteins. Recovery was no less than 80%, about 1-3 mg per 10 mL serum, obtaining C4-A3, A4, A6, and C4-Bl/B2.
Creative Biolabs has long-lasting expertise in designing and conducting the purification of complement components. With years of experience, now we can provide a series of high-quality products and services for our global clients. We are confident in offering substantial help and advice on your project.
To learn more details, you can get access with the following links:
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Complement Products
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Complement Test Services
If you are interested in our products and services, please do not hesitate to contact us for more information.
Please note that our protocols are only for your reference!
For Research Use Only.
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