C4B

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Host Animal Rat
Specie Reactivity Mouse
Antibody Clone CTJS-861
Isotype IgG2a
Applications WB; IF; IHC; IP
Host Animal Mouse
Specie Reactivity Human
Antibody Clone CTJS-456
Isotype IgG2b Kappa
Applications WB; ELISA; ICC
Host Animal Mouse
Specie Reactivity Human
Antibody Clone CTJS-523
Isotype IgG2b Kappa
Applications WB; ELISA
Host Animal Chicken
Specie Reactivity Human
Isotype IgY
Applications WB; ELISA; FC; IHC
Host Animal Rabbit
Specie Reactivity Human
Isotype IgG
Applications WB
Host Animal Mouse
Specie Reactivity Human
Antibody Clone TJL-45
Isotype IgG1
Applications ICC/IF
Host Animal Mouse
Specie Reactivity Human
Antibody Clone TJL-46
Isotype IgG1
Applications IF
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Specie Reactivity Human
Sensitivity 3.9 ng/mL (15.6-1000 ng/mL)
Applications ELISA
Specie Reactivity Human
Sensitivity 0.55 ng/mL (1.25-80 ng/mL)
Applications ELISA
Specie Reactivity Human
Sensitivity 3.9 ng/mL (15.6-1000 ng/mL)
Applications ELISA
Specie Reactivity Mouse
Sensitivity 4.79 ng/mL (15.625-1000 ng/mL)
Applications ELISA
Specie Reactivity Mouse
Sensitivity 0.72 ng/mL (1.56-100 ng/mL)
Applications ELISA
Specie Reactivity Rat
Sensitivity 25.6 pg/mL (62.5-4000 pg/mL)
Applications ELISA
Specie Reactivity Rat
Sensitivity 1.0 ng/mL (2.5-50 ng/mL)
Applications ELISA
Specie Reactivity Rabbit
Sensitivity 1.0 ng/mL (50-1000 ng/mL)
Applications ELISA
Specie Reactivity Chicken
Sensitivity 0.141 ng/mL (0.234-15 ng/mL)
Applications ELISA
Specie Reactivity Porcine
Sensitivity 2.813 ng/mL (4.688-300 ng/mL)
Applications ELISA
Specie Reactivity Guinea Porcine
Sensitivity 1.0 ng/mL (50-1000 ng/mL)
Applications ELISA
Specie Reactivity Monkey
Sensitivity 1.0 ng/mL (2.5-50 ng/mL)
Applications ELISA
Specie Reactivity Canine
Sensitivity 1.0 ng/mL (50-1000 ng/mL)
Applications ELISA
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Specie Reactivity Human
Type Recombinant Protein
Expression Host Wheat Germ (in vitro)
Applications WB; ELISA; PA; AP
Specie Reactivity Human
Type Recombinant Protein
Expression Host Wheat Germ (in vitro)
Applications WB; ELISA; PA; AP
Specie Reactivity Human
Type Recombinant Protein
Expression Host E. Coli
Applications Positive Control; Immunogen; WB
Specie Reactivity Mouse
Type Recombinant Protein
Expression Host E.coli/Mammalian cell/Baculovirus
Applications WB; ELISA
Specie Reactivity Human
Type Native Protein
Applications Functional Assays
Specie Reactivity Human
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA; IP
Specie Reactivity Human
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA
Specie Reactivity Mouse
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA; IP
Specie Reactivity Mouse
Type Recombinant Protein
Expression Host E.coli
Applications WB; ELISA; IP
Specie Reactivity Mouse
Type Recombinant Protein
Expression Host Yeast
Applications WB; ELISA
Specie Reactivity Human
Purity >90%
Applications IHC; WB
Specie Reactivity Human
Purity >90%
Applications Blocking; IHC; WB
Specie Reactivity Human
Purity >90%
Applications Blocking
[Cat#: CTApt-724] Anti-C4B Aptamer SELEX Service
Affinity (Kd) 1nM~1μM
Chemical Modification N/A
Clear All
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-AC-GFP
Tag GFP
Specie Reactivity Human
Type Expression Plasmid
Vector pCMV6-Entry
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-AC-GFP
Tag GFP
Specie Reactivity Mouse
Type Expression Plasmid
Vector pCMV6-Entry
Specie Reactivity Rat
Type Expression Plasmid
Vector pCMV6-Entry
Tag Myc-DDK
Specie Reactivity Rat
Type Expression Plasmid
Vector pCMV6-Entry
Product List Background

Background

Normally, when the complement classical pathway was activated by the immune complex or the lectin pathway was activated by glycoprotein components on pathogen membrane surfaces, the proteases C1s and MASP-2 were activated, respectively. C1s and MASP-2 cleave the complement component 4 (C4) alpha chain into C4a and C4b fragments. C4a serves as an anaphylatoxin involved in local inflammation. C4b is an important member of the complement cascade that participates in the formation of the C3 convertases (C4bC2b) and C5 convertases (C4bC2bC3b).

Human C4b is a glycosylated polypeptide with 188 kDa, containing 12 domains (eight macroglobulin domains, a thioester domain, a CUB domain, fibronectin type III domain, and a C345C C-terminal domain). The cleavage of C4 into C4b is realized by the removal of the ANA domain, also named as C4a fragment. The activated C4b can covalently attach to hydroxyl or amine groups on pathogenic or target surfaces, forming the C3/C5 convertase enzyme complex. Surface-bound C4b has binding sites for the CCP module containing complement regulatory proteins, including C4b binding protein (C4BP), complement receptor 1, membrane cofactor protein, decay accelerator factor, and factor I. When binds to the complement regulatory proteins, C4b will be cleaved into C4c and C4d, resulting in loss of complement activation activity. The abnormality of C4b is related to complement dysfunction, immunodeficiency, and systemic lupus erythematosus.

The structural changes accompanying activation of C4 and its transition to C4b.Fig. 1 The structural changes accompanying activation of C4 and its transition to C4b.1, 2

References

  1. Mortensen, Sofia, et al. "Structural basis for the function of complement component C4 within the classical and lectin pathways of complement." The Journal of Immunology 194.11 (2015): 5488-5496.
  2. under Open Access license CC BY 4.0, without modification
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