Lactobacillus sakei-derived Exosome Research and Application
Lactobacillus sakei, a type of probiotic, belongs to the lactic acid bacteria family. It is commonly found in the fermentation processes of various foods, including fermented meat products, dairy products, and vegetable-based foods. Lactobacillus sakei has gained attention for its numerous advantages. Recently, scientists have shown an increasing interest in studying exosome, and they have identified exosomes as a potential application area for Lactobacillus sakei. Creative Biolabs has initiated a microbial-derived exosome development platform, providing a unique opportunity for customers to explore and develop innovative microbial-derived exosomes.
Various Applications of Lactobacillus sakei
1. Gut Health: Lactobacillus sakei can promote a balanced gut microbiota by increasing the number of beneficial bacteria and inhibiting the growth of harmful bacteria, thereby improving gut health. This is crucial for maintaining normal immune system function and reducing gastrointestinal issues. Several studies have reported the improvement of colitis by Lactobacillus sakei.
2. Food Fermentation: Lactobacillus sakei finds wide application in food fermentation. It can alter the flavor and texture of foods while also producing antibacterial substances that help extend the shelf life of food products.
3. Antimicrobial Properties: Lactobacillus sakei can produce various antimicrobial substances, such as bacteriocins, which can inhibit the growth of various pathogenic bacteria, including Listeria monocytogenes and Escherichia coli.
4. Customizability: Lactobacillus sakei strains can be selected and improved to meet specific application requirements, increasing the flexibility of its use.
Prospects for Lactobacillus sakei-Derived Exosome
Exosomes are microvesicular structures containing various bioactive molecules derived from the host. Due to the exceptional properties of Lactobacillus sakei, Lactobacillus sakei-derived exosomes have a broad range of potential applications, including but not limited to the following areas:
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Gut Health: Lactobacillus sakei-derived exosomes may carry bioactive molecules that contribute to maintaining gut health. These exosomes can be used as prebiotics, helping to improve the balance of the gut microbiota, reduce inflammation, and promote gut health. By promoting the growth of beneficial bacterial populations and combating pathogenic microbes, they have the potential to play a positive role in the prevention and treatment of gastrointestinal diseases.
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Immune Regulation: Components in Lactobacillus sakei-derived exosomes may possess immune-regulatory capabilities. By stimulating host immune responses, exosomes have the potential to enhance immune system efficiency and reduce the risk of immune-related diseases.
Of course, these prospects require further in-depth research and experimental validation to fully understand the potential value of Lactobacillus sakei-derived exosomes in these application areas.
Research Findings on Lactobacillus sakei-Derived Exosomes
Regarding the application of immune regulation, research has revealed the potential role of Lactobacillus sakei-derived exosomes in regulating the intestinal immune system, particularly concerning the production of immunoglobulin A (IgA) and intestinal immune function.
Lactobacillus sakei-derived exosomes stimulated IgA production in Peyer's patch lymphoid follicle cells in mice. This stimulatory effect can be weakened by inhibiting Toll-like receptor 2 (TLR2) neutralization since TLR2 can recognize components of Gram-positive bacterial cell walls. This result suggests that Lactobacillus sakei-derived exosomes play a crucial role in maintaining intestinal immune balance and preventing the invasion of pathogenic microorganisms.
Two years later, the same Japanese research team further explained that Lactobacillus sakei-derived exosomes induced the production of nitric oxide (NO) and retinoic acid (RA) in Peyer's patch lymphoid follicle cells by upregulating the expression of inducible nitric oxide synthase (iNOS) and retinaldehyde dehydrogenase 2 (RALDH2) genes. This induction promoted the production of IgA. The study also revealed that bone marrow-derived dendritic cells produced interleukin-6 (IL-6) in response to exosome stimulation, and IL-6 promoted the differentiation of B cells into plasma cells, further enhancing IgA production in Peyer's patch lymphoid follicle cells. All of these findings demonstrate that Lactobacillus sakei-derived exosomes strengthen the intestinal immune response by promoting IgA production in Peyer's patch cells.
Creative Biolabs, a highly regarded biotechnology company, specializes in the field of exosome research and development, offering a diverse range of exosome technology services. We eagerly look forward to collaborating with customers to advance the development of microbial-derived exosomes. If you need more information or are interested in partnership opportunities, please feel free to contact us.
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In Vivo Functional Discovery of Bacteria-derived Exosomes
References
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Yamasaki-Yashiki, S.; Miyoshi, Y.; et al. IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893. Bioscience of Microbiota Food and Health. 2019. 38(1):23-29.
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Miyoshi, Y.; Saika, A.; et al. Mechanisms underlying enhanced IgA production in Peyer's patch cells by membrane vesicles derived from Lactobacillus sakei. Bioscience Biotechnology and Biochemistry. 2021. 85(6):1536-1545.
For Research Use Only. Cannot be used by patients.
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