Propionibacterium freudenreichii-derived Exosome Research and Application

The probiotic Propionibacterium freudenreichii has been found to have anti-inflammatory effects in several studies, and further exosomes isolated from it have been shown to contain a wide range of immunomodulatory proteins capable of interacting with the host's immunomodulatory factors and thus exporting inflammation-regulating functions. Creative Biolabs has knowledge of the Propionibacterium freudenreichii-derived exosomes and is capable of providing reliable services to aid in the study of Propionibacterium freudenreichii-derived exosomes.

Propionibacterium freudenreichii-derived Exosome Isolation

Culture Propionibacterium freudenreichii under suitable liquid conditions to stationary phase.
Collect and centrifuge the bacteria-free culture of the solid phase culture strain to precipitate residual bacteria and collect the supernatant of Propionibacterium freudenreichii.
Filter the strain supernatant fraction to further remove Propionibacterium freudenreichii cells.
Concentrate the supernatant by ultrafiltration followed by serial centrifugation and recover the concentrated exosomes with TBS buffer.
Purifying Propionibacterium freudenreichii exosomes further by size-exclusion chromatography.
Discard protein-contaminated and ineffective fractions and finally pool fractions containing Propionibacterium freudenreichii exosomes.

Research on Propionibacterium freudenreichii-derived Exosomes

Research	Conclusion
Protein analysis of Propionibacterium freudenreichii-derived exosomes.	The cargo proteins of exosomes were analyzed by nanoLC-ESI-MS/MS, and it was found that Propionibacterium freudenreichii-derived exosomes carry functionally diverse proteins. Among them, immunomodulatory proteins such as Surface layer protein SlpB, enolase 1, iron/manganese superoxide dismutase, and malate dehydrogenase showed high abundance.
Propionibacterium freudenreichii-derived exosomes regulated the NF-κB pathway and pro-inflammatory factor release.	In vitro experiments demonstrated that Propionibacterium freudenreichii-derived exosomes were able to dose-dependently reduce the amount of NF-κB activator in lipopolysaccharide-induced human intestinal epithelial cells, thereby exerting an attenuating effect on the inflammatory response. In the lipopolysaccharide-induced inflammation model of human intestinal epithelial cells, it was observed that treatment of Propionibacterium freudenreichii-derived exosomes reduced the pro-inflammatory factor IL-8 to non-inflammatory control levels. Cell proliferation assays showed that these changes were caused by Propionibacterium freudenreichii-derived exosomes exerting regulatory activity rather than producing cytotoxicity.
Surface layer proteins are involved in the anti-inflammatory properties of Propionibacterium freudenreichii-derived exosomes.	Comparing the effects of exosomes derived from genetic mutant strains revealed that SlpB is one of the effectors involved in the regulation of the NF-κB pathway by the Propionibacterium freudenreichii exosomes, whereas there may be other effectors involved as well.

Applications of Propionibacterium freudenreichii-derived Exosomes

Propionibacterium freudenreichii exosomes can be the research subject to help explain the molecular mechanisms underlying the probiotic properties of the organism from which they are derived.
Propionibacterium freudenreichii exosomes can be used as bioactive agents to advance the development of therapeutic products for inflammation-related diseases.
Propionibacterium freudenreichii exosomes are one of the commensal bacteria in the human gastrointestinal tract and can be developed as agents that modulate the ecology of the digestive flora.
Propionibacterium freudenreichii-derived exosomes can also be involved in the processing and flavor modulation of food products such as cheese, as can their strains.

Fig. 1 Characterization of the morphology and size for Propionibacterium freudenreichii-derived exosomes.¹

The understanding of the characterization and function of Propionibacterium freudenreichii-derived exosomes facilitates further research and understanding of Propionibacterium freudenreichii-derived exosome activities and mechanisms. Creative Biolabs has a professional exosome research platform to promote the application and development of Propionibacterium freudenreichii-derived exosomes. Please contact us to discuss.

Reference

Rodovalho, Vinícius de Rezende, et al. "Extracellular vesicles produced by the probiotic Propionibacterium freudenreichii CIRM-BIA 129 mitigate inflammation by modulating the NF-κB pathway." Frontiers in microbiology 11 (2020): 1544.

Plant Exosome Isolation