Stem Cell-derived Exosome Application
- Synovial Mesenchymal Source
Overview Services Features FAQs
The application of SMSC-Exo (Synovial mesenchymal stem cell-derived exosomes) is closely related to the intervention of arthritis by promoting chondrocyte regeneration, improving cartilage matrix homeostasis, and relieving pain, thereby influencing the fate of joint tissue. Creative Biolabs reviews the functions of SMSC-Exo and offers research services to provide new perspectives and strategies for better application in arthritis.
Multiple Applications of SMSC-Exo in Arthritis
Protection of chondrocytes
SMSC-Exo can be endocytosed and aggregated intracellularly after co-culture with chondrocytes. SMSC-Exo overexpressing miR-140-5p can target and inhibit RalA to rescue the chondrogenic differentiation factor SOX9. Cell viability and migration assays confirmed the pro-proliferative and migratory effects of SMSC-Exo. Meanwhile, cell growth factor TGFβ1 and proliferating cell nuclear antigen were upregulated, and apoptosis marker CASP3 was downregulated.
Mitigation of cartilage and matrix degradation
SMSC-Exo treatment of a rat osteoarthritis model induced by sodium iodoacetate upregulated in cartilage tissue the collagen type II alpha 1 chain protein and downregulated the metalloproteinase 13 protein responsible for degradation of collagen and proteoglycan in the matrix. miR-320 overexpression in SMSC-Exo further amplified the repair of the cartilage matrix by directly inhibiting a disintegrin and metalloproteinase 19 to further amplify the repair of the cartilage matrix.
Anti-inflammatory
miR-129-5p, shuttled by SMSC-Exo, targeted and inhibited High Mobility Group Protein 1 and significantly attenuated the pro-inflammatory factors IL-1β, IL-6, and TNF-α in an IL-1β-induced arthritis model. Histological analysis revealed downregulation of calcitonin gene-related peptide and inducible nitric oxide synthase in rat knee specimens and dorsal root ganglia. In addition, SMSC-Exo-carrying circEDIL3 served as the sponge for miR-485-3p targeting PIAS3 to alleviate the rheumatoid arthritis mouse model.
Alleviation of cartilage pain
The pathophysiology of arthritic pain involves nociceptive hyperalgesia and aberrant pain, which arise from the central and peripheral sensitization of neuropathic pain. SMSC-Exo alleviated pain-related behavioral changes in intra-articular sodium iodoacetate injection and partial medial meniscectomy knee models, as evidenced by restoration of mechanical claw reduction thresholds and thermal claw reduction latencies to normal.
Rescue of cartilage damage
Tissue staining of knee specimens collected from animals showed disorganized arthritic cartilage structures with rough and fractured surfaces. Some of the subchondral bone was exposed, the synovial membrane was congested, and multiple bony redundancies were formed. In contrast, SMSC-Exo treatment in the cartilage showed a small number of defects and fractures, indicating a reduction in cartilage damage.
Services Offered at Creative Biolabs
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Exosome Isolation and Characterization
Isolation: We offer cutting-edge methods, including ultracentrifugation, for the isolation of exosomes produced from synovial mesenchymal stem cells.
Characterization: Our comprehensive characterization services include NTA, TEM, and WB to confirm exosome purity and analyze their protein content.
Biological Activity Assessment: Evaluate the therapeutic potential of synovial MSC-derived exosomes through functional assays such as cell migration, proliferation, and anti-inflammatory assays.
Targeted Delivery Studies: Assess the capability of exosomes to target specific tissues or cells and their efficacy in delivering bioactive molecules.
Customized Exosome Preparation: Tailor the exosome preparation process to meet specific research needs, including modification with fluorescent labels or agents.
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Data Analysis and Reporting
In-depth Analysis: Provide detailed analysis of experimental data, including statistical evaluations and graphical representations.
Reporting: Deliver comprehensive reports with actionable insights and recommendations based on the findings.
SMSC and Exosomes in Relation to Arthritis
Intra-articular injection of SMSC has the potential for arthritis treatment. Thanks to their paracrine mechanism, SMSC-Exos exert a similar effect and show additional advantages.
Fig. 1 Current cell-based and cell-free therapies for osteoarthritis.1
SMSC-Exos in arthritis exhibit the potential for promoting cartilage repair as well as reducing inflammation and pain in the knee. Creative Biolabs provides one-stop services to support the research and application of SMSC-Exo. Please contact us to inform your requirements.
FAQs
Q: Can you customize the exosomes for specific applications?
A: Yes, we offer customization services, including the incorporation of fluorescent labels or therapeutic agents into the exosomes. We can also tailor the isolation and formulation processes to suit your research needs.
Q: What are the typical applications of stem cell-derived exosomes from synovial mesenchymal sources?
A: Stem cell-derived exosomes from synovial mesenchymal sources are explored for various applications, including regenerative medicine, anti-inflammatory therapies, and cosmetic enhancements. When it comes to musculoskeletal disorders, they are very helpful in addressing joint tissues and accelerating healing.
Q: What handling and storage procedures are advised for exosome samples?
A: Liquid exosome samples should be stored at -80°C to maintain their stability. For shipping, we use dry ice to ensure that liquid exosomes remain frozen during transit. Preserving the integrity of the exosomes requires careful handling and preservation.
Reference
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Zhang, Zehao, et al. "Enhancement of the therapeutic efficacy of mesenchymal stem cell-derived exosomes in osteoarthritis." Cellular & Molecular Biology Letters 28.1 (2023): 75. Under open access license CC BY 4.0. The image was modified by revising the title.
For Research Use Only. Cannot be used by patients.
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