Antibody Glycoprofiling Service
Services Published Data FAQs Customer Review
Antibody Glycoprofiling Service at Creative Biolabs
The glycosylation pattern of an antibody is a key quality attribute, which has an important impact on the structure, safety, stability, and immunogenicity of the antibody. Comprehensive glycosylation analysis of antibodies helps evaluate the impact of different glycosyl structures on antibody functions and activity. Creative Biolabs has developed a multifunctional Glycoprotein Analysis Platform. We use various mass spectrometry techniques to comprehensively identify the glycoforms of antibodies and conduct professional glycosylation profile assessments of Fab and Fc domains.
Glycoprofiling analysis of antibodies is generally performed according to the following steps.
We collect and extract antibody samples, and perform protein purification and enrichment processing on the samples to reduce the impact of interfering substances on glycosylation analysis and eliminate non-specific signals.
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Enzymatic digestion reaction
We add an appropriate enzyme (such as PNGase F) to the antibody sample and incubate it for several hours at 37 °C to remove glycosylation. The released glycans are then labeled.
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Chromatographic separation
We use liquid chromatography technology or capillary electrophoresis to elute and separate labeled glycans and calculate the relative glycan composition.
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Mass spectrometry (MS) analysis
To correctly identify the labeled glycans, we couple the separation system to an MS system and use positive ion mode to collect precursor ions and fragment product ions of the sample. Moreover, we analyze the glycan composition in the antibody sample and generate detailed reports according to the detected mass spectrometry data, including glycan type, relative abundance, possible modification types, etc., thereby providing clients with comprehensive analysis results on the antibody glycans.
Fig.1 The workflow of antibody glycoprofiling.
Published data
The glycosylation pattern of IgG1 Fc affects the effector functions of IgG and is closely related to aging. In this study, the authors compared the glycosylation pattern of total anti-spike (S) IgG1 produced by healthy controls (HC) and patients with Down syndrome (DS) before and after SARS-CoV-2 vaccination. They used liquid chromatography-mass spectrometry (LC-MS) technology to detect the glycan profile of IgG1 Fc N-glycans in both groups before and after vaccination. The results showed that compared with HC, the levels of sialylation and galactosylation decreased with age in patients with DS, while fucosylation increased with age. In addition, the concentration of anti-S antibodies in patients with DS was reduced after vaccination. These findings provided new insights into our understanding of the susceptibility of patients with DS to severe infections and helped develop specialized vaccination strategies for this population.
Fig.2 Antibody Fc N-glycosylation and antibody glycomics workflow.1
Advantages
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We provide glycoprofiling for various biological samples, such as antibodies, Serum, Cell Tissues, Urine, etc.
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We have established a complete standardized operating process to ensure the accuracy and reliability of the glycoprofiling analysis process.
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We promptly respond to client needs and provide efficient sample processing and glycoprofiling analysis services.
Application
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Biological stability assessment: Analyzing the glycosyl structure in antibody samples evaluates the impact of glycosyl on the biological stability and half-life of the antibody in vivo, helping to optimize the stability of the antibody.
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Pharmaceutical research: Analysis of the glycosyl structure of antibodies helps to understand the pharmacodynamic properties of antibodies, including its impact on receptor binding affinity and activity, as well as the impact of glycosyl on the metabolism and clearance rate of antibodies in the body.
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Drug development: Antibody glycosylation analysis provides important information for drug development, which is used to help optimize antibody drug design.
Creative Biolabs has experienced scientific research teams dedicated to providing global clients with a wide range of antibody glycoprofiling analyses. Whether you use glycoprofiling for research, development, or quality control, you can trust us to provide quality glycoprofiling services tailored to your specific needs. Please feel free to contact us if you are interested in our glycoprofiling analysis.
FAQ
Q1: What is the impact of antibody glycosylation? Why do we perform antibodies glycoprofiling?
A1: Glycosylation of antibodies has an important impact on their structure, stability, safety, and immunogenicity. Different glycosylation structures change the biological functions of antibodies, such as affecting the binding affinity to receptors and the metabolic rate in the body. Glycoprofiling of antibodies helps researchers gain a deeper understanding of the specific effects of glycosylation on antibody efficacy, thereby optimizing antibody design, and improving the efficacy and safety of drugs, and is essential for drug development and quality control.
Q2: How should I prepare samples for antibodies glycoprofiling?
A2: When preparing antibody samples for glycoprofiling, first ensure that the sample is prepared under proper conditions. Next, appropriate protein purification and enrichment procedures are used to remove potentially interfering substances, such as salts and impurities, to reduce the impact on subsequent glycosylation analysis. During the processing, the stability of the samples needs to be maintained. Finally, the processed samples are sent to our laboratory for subsequent analysis. Moreover, you also send the samples directly to our laboratory, and we will perform a series of pre-treatment steps.
Q3: What technologies can be used for antibodies glycoprofiling?
A3: Glycoprofiling of antibodies is performed using a variety of techniques, including MS, liquid chromatography (LC), capillary electrophoresis (CE), etc. MS provides detailed information on sugar composition by identifying and quantifying glycosylation products, LC is used to separate different sugar structures to improve the accuracy of analysis, and CE is suitable for rapid analysis of small amounts of samples. These techniques are applied in combination to ensure a comprehensive and efficient analysis of antibody glycosylation.
Customer Review
The Analysis Results Are Very Detailed and Clear!
“Creative Biolabs classified the relative abundance of different glycan types in detail in the report, allowing us to clearly understand the glycosylation status of the antibody, providing valuable data support for our subsequent research and development.”
Helped Us Optimize Antibody Drug Design!
“Creative Biolabs conducted a comprehensive and in-depth glycosylation pattern analysis of our antibody samples, which helped us successfully adjust the antibody design and improve the stability and efficacy of the drug. We plan to continue to choose Creative Biolabs for glycoprofiling in future projects.”
Reference
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Streng, Bianca MM, et al. "IgG1 glycosylation highlights premature aging in Down syndrome." Aging Cell (2024): e14167. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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