Glycan Modification and Labeling Services

Services Published Data FAQs Customer Review

To create derivatives with determined/adapted properties of glycan, more and more researchers pay attention to glycan modification and labeling. As a leading global company, Creative Biolabs has developed a cutting-edge platform with advanced technologies and experienced scientists specialized in the study of carbohydrates. Based on this platform, we are capable of providing high-quality glycan modification and labeling services to meet our global customers' every specific demand.

Glycan Modification and Labeling

Glycans have many highly reactive groups (acetamido, amino, carboxyl, and hydroxyl groups) that can be exploited in various functionalization pathways. In particular, they are characterized by high hydrophilicity due to the presence of many hydroxyl groups on their structure. Due to their diversity of structures and their properties, glycan constitutes interesting renewable sources that can be used as high-performance material. Most of them are biologically inert, safe for humans, and abundantly available in the natural environment. They have several other advantages such as low costs, biodegradability in the ecosystem, renewability, polyfunctionality, chemical reactivity, chelating and absorptive capacities in the treatment of wastewater. Environmental concerns and sustainability have become key issues today. In this context, there is an increased interest in the industrial use of renewable resources such as starch, chitosan, cellulose, and pectin. Efforts are made in the research and the development of glycan derivatives as basic materials for new applications based on new and/or improved properties of these biopolymers. Over the years, many chemical reactions such as biotinylation, sulfation, PEGylation, phosphorylation, acetylation, have been performed involving the free carboxyl, amino or hydroxyl groups distributed along the polysaccharide backbone aiming to create derivatives with determined/adapted properties.

Glycan Modification and Labeling Services at Creative Biolabs

As an industry-leading global company, Creative Biolabs has established a comprehensive platform with advanced technologies and high-level scientific research team specialized in glycan modification and labeling. Our Ph.D. level scientists offer tailored experimental schemes for global customers according to their specific study purpose. Our platform provides various types of glycan modification and labeling services including but not limited to fluorescence labeling, biotinylation service, PEGylation, isotope labeling, sulfate modification, phosphorylation modification, acetylation modification, and berberine labeling research.

Advantages of Our Service Including but Not Limited to:

Professional: Creative Biolabs owns experienced scientists specialized in glycan modification and labeling.
Reliable: Creative Biolabs has established advanced technology platforms and accomplished hundreds of projects.
Customers-first: Creative Biolabs provides efficient, accurate, and cost-efficient services.

As a leading global CRO company, Creative Biolabs is committed to providing high-quality glycan modification and labeling services to researchers around the world. We are more than happy to share our experience and help our customers with this extremely important step in glycan related research. Please feel free to contact us for more information.

Published data

Mucin-type O-glycans are sugar groups connected to serine or threonine residues through O-linkages. They usually have complex core polysaccharide chains and highly branched structures, which contain a variety of chemical modifications such as sulfation. These characteristics enable them to play a key role in the body. In this study, the authors developed a new process for analyzing mucin-type O-glycomics. They released O-glycans from glycoproteins by non-reductive β-elimination and then labeled the ProA fluorescent tag at the GalNAc-reducing end of the glycan core. Next, hydrophilic interaction liquid chromatography was used to separate ProA-labeled O-glycans, and the composition and relative abundance of O-glycans were accurately quantitatively analyzed by fluorescent labeling. Moreover, the authors applied this method to the analysis of mucins from different tissue types and mammals, and the results showed the wide applicability of this analytical method.

Fig.1 Labeling and analysis of mucin-type O-glycans. Fig.1 Fluorescent labeling and analysis of O-glycans.¹

FAQ

Q1: How do you handle the purification and characterization of modified glycans?

A1: We employ state-of-the-art purification techniques such as chromatography methods (HPLC, SEC) followed by detailed characterization using techniques like mass spectrometry (MS), nuclear magnetic resonance (NMR), and high-resolution microscopy. This ensures the purity and precise structural characterization of modified glycans.

Q2: What types of glycan modifications do you specialize in?

A2: At Creative Biolabs, we specialize in a variety of glycan modifications including biotinylation, fluorescence labeling, isotope labeling, PEGylation, acetylation modification, phosphorylation modification, sulfate modification, etc. Of course, we also provide tailor-made experimental plans according to the specific research objectives of our clients. Our experienced scientists will work closely with you to design and implement appropriate glycan modification and labeling strategies to meet your specific project needs.

Q3: How can I get a quote or more information about your glycan modification and labeling services?

A3: You can easily get a quote or more information by contacting us directly via email or through our website. Our client service team is prompt and will connect you with our scientists to discuss your research goals and help design the most appropriate modifications and labeling strategies.

Customer Review

High-precision Biotin Labeling
“Creative Biolabs provided an outstanding service for our glycan biotinylation project. Their team ensured that the biotin labeling was performed with high precision, and the resultant glycan derivatives showed excellent binding affinity in our experiments. The comprehensive report they provided also helped us understand the modifications better.”

Stable Glycan Fluorescent Labeling
“Our project required fluorescence labeling of polysaccharides, and Creative Biolabs delivered beyond our expectations. The labeled glycans exhibited strong and stable fluorescence signals, which enhanced our imaging studies significantly. The turnaround time was also impressive.”

Reference

Safferthal, Marc, et al. "Labeling of Mucin-Type O-Glycans for Quantification Using Liquid Chromatography and Fluorescence Detection." ACS Measurement Science Au 4.2 (2024): 223-230. Distributed under Open Access license CC BY 4.0, without modification.