Serum Glycoprofiling Service

Accurate and Reliable Serum Glycoprofiling Service at Creative Biolabs

The type and abundance of glycans in serum are closely related to the occurrence of diseases. Creative Biolabs provides professional serum glycoprofiling services, which help provide researchers, doctors, and patients with important bioinformatics data, thereby helping to promote research and clinical applications in the field of life sciences.

• Mass spectrometry-based serum glycoprofiling service
  Serum is an easy-to-sample biological sample that contains high concentrations of glycoproteins. We perform glycoprofiling of serum using mass spectrometry and high-performance liquid chromatography.
  • Sample collection: We collect serum samples to be tested and store them at -20°C.
  • Sample pre-processing: We perform necessary pre-processing steps on samples according to detection needs, such as protein removal, enrichment, etc., to reduce possible interfering substances.
  • Release and labeling of glycans: We mix serum, water, and PNGase F buffer and incubate under appropriate temperature conditions to quickly release glycans from the serum. Moreover, the released glycans are also labeled.
  • Glycan elution and analysis: We place labeled samples in a liquid phase analysis system, optimize gradient elution conditions, and separate the glycans in the samples. In addition, we determine the structure of the glycans by exoglycosidase sequencing of labeled glycan libraries and liquid chromatography-mass spectrometry analysis.
• Lectin microarray-based serum glycoprofiling service
  Serum glycoprofiling using lectin microarrays achieves high-throughput detection and identification of multiple sugars in serum samples. The following are general steps for serum glycoprofiling using lectin microarrays.
  • Preparation of lectin microarrays: We select lectins with affinity and immobilize them on the microarray, such as peanut agglutinin (PNA).
  • Sample processing: We collect serum samples and perform appropriate pre-processing steps to reduce the impact of interfering substances on subsequent analysis.
  • Sample incubation and washing: We add the pre-processed serum samples to the lectin microarray to allow the sugars in them to bind to the lectins. This is followed by a washing step to remove non-specifically bound components.
  • Fluorescent labeling: We then use fluorescently labeled probes to label the sugars that bind to the lectin and form complexes.
  • Data analysis: We use an imaging system to acquire fluorescence signal images on the lectin microarray and perform quantitative analysis on each spot. The results are interpreted using image processing and data analysis software to determine the content and structure of different sugars.

Published data

Abnormal glycosylation is an important hallmark of cancer. In this study, the authors used serum samples from healthy individuals as a control group to study the O-glycosylation characteristics of apolipoprotein C-III (apoC-III) in the serum of colorectal cancer (CRC) patients, thereby evaluating the differences in O-glycosylation of apoC-III in CRC patients. The authors directly analyzed the O-glycosylation of apoC-III protein in serum samples using a simple positive ion mode matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique. This analysis process did not require specific protein separation, was relatively simple to operate, and achieved high-throughput analysis. The results showed that compared with the control group, the O-glycosylation of apoC-III in the serum samples of CRC patients changed significantly, especially the sialylation level was significantly increased.

Fig.1 O-glycosylation analysis of apoC-III in two groups of serum samples.¹

Advantages

• Professional team: We have experienced scientists and technicians who perform sample processing and data analysis accurately and efficiently.
• Complete technology: We have complete laboratory equipment and technology, providing high-sensitivity and high-resolution serum glycoprofiling analysis.
• Diversified analysis technologies: We provide diversified analysis technologies to provide comprehensive support for serum glycoprofiling analysis.

Application

• Disease monitoring: Sugar molecules in serum play a vital role in the occurrence of diseases. Serum glycoprofiling analysis can discover carbohydrate markers in specific disease states, assisting in early diagnosis of the disease, monitoring of disease progression, and evaluation of treatment effects.
• Evaluation of drug efficacy: Sugar molecules in serum reflect the effect of drugs on the body. Serum glycoprofiling analysis evaluates the effectiveness and safety of drugs and guides the development of personalized treatment plans.
• Nutrition and health assessment: Serum glycoprofiling analysis is also used to assess an individual's nutritional status and metabolic health, providing data support for personalized health management.

Creative Biolabs has profound technical accumulation in the field of Glycoprotein Analysis. We have established a comprehensive automated analysis platform to support glycoprofile analysis of different biological samples, such as Antibody, serum, Plasma, etc. We provide clients with accurate and reliable serum glycoprofiling analysis services and help clients carry out related research and applications. Please feel free to contact us if you would like to get detailed analysis content.

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Reference

1. Kianičková, Kristína, et al. "O-glycoprofiling of Serum Apolipoprotein C-III in Colorectal Cancer." Frontiers in Bioscience-Landmark 29.1 (2024): 32. Distributed under Open Access license CC BY 4.0, without modification.

Related Services

• Glycoprotein Detection
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• Quantitative Sialic Acid Analysis
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