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Glycosylation serves essential functions on many proteins especially therapeutic antibodies produced in biopharmaceutical manufacturing, making it mandatory to thoroughly consider its biogenesis during the production process. However, protein glycosylation and synthesis of the glycosaminoglycan (GAG) are non-templated, and thus, significant heterogeneity can arise from organism to organism, cell type to cell type, and even between different culture conditions.
A variety of different production systems such as mammalian cells, yeast cells, and plant cells, are currently available for recombinant glycoprotein production purposes. Creative Biolabs has successfully developed our own glyco-engineered systems optimized for improving the productivity of glycoprotein, process robustness, and reducing cell line generation timelines. We offer high-quality customized services by adjusting strategies to meet even the most specific requirements in the production of glycoprotein.
Fig.1 Glycoforms of concern in different systems.1, 3
Correct glycan structures are crucial for the pharmacokinetic and pharmacodynamic properties of glycoprotein biologics and therapeutic carbohydrates. Hyperglycosylated proteins show increased serum half-life and are more heat-stable and less sensitive to proteolysis compared with the non-glycosylated forms. Different expression platforms are actively pursued by many academic and industrial laboratories. With years of research and experience in mammalian cell glycoengineering, Creative Biolabs has developed the following glyco-engineered systems for improved glycoprotein production:
Creative Biolabs is committed to providing optional glyco-engineered expression systems for therapeutic glycoprotein development and high-quality glycoprotein products to promote the progress of your projects. Please feel free to contact us for more information and a detailed quote.
Plant systems are an attractive host for producing recombinant monoclonal antibodies with well-defined glycosylation. In the present study, the researchers expressed a recombinant human IgA1 transiently in Nicotiana benthamiana. Analysis of its glycosylation revealed the presence of plant-specific modifications on IgA1, including pentose attachment and hydroxyproline formation. IgA1 also had complex biantennary N-glycans with terminal N-acetylglucosamine. This study demonstrates the feasibility of using plants to produce recombinant monoclonal antibodies with defined human-type O- and N-linked glycans and provides a model for our construction of a glycol-engineering system for producing therapeutic glycoproteins.
Fig.2 Constructs for expression and glyco-engineering.2, 3
Q1: What is the impact of glycol-engineering on the efficacy and safety of therapeutic glycoproteins?
A1: Glyco-engineering has been used to optimize the stability and biological activity of therapeutic glycoproteins. By adjusting glycosylation sites or glycosylation profiles, the stability, transport, half-life, efficacy, and immunogenicity of therapeutic glycoproteins can be optimized.
Q2: What are the advantages of mammalian cell systems?
A2: The mammalian cell system has complex N-type glycosylation and accurate O-type glycosylation post-translational processing. The final recombinant glycoproteins produced are close to natural glycoproteins in terms of molecular structure, physicochemical properties, and biological functions. This feature makes mammalian cell systems widely used in the development and production of recombinant therapeutic glycoproteins.
High-quality Glyco-engineered System
"Our experience with the glyco-engineering system provided by Creative Biolabs was very good. The team demonstrated solid expertise and extensive experience in this area. The final recombinant therapeutic glycoproteins produced were much more stable. We were very satisfied with the quality of the entire service and the final result. We appreciated the team's efforts in optimizing our therapeutic glycoproteins through glyco-engineering."
Glyco-engineering System That Exceeds Expectations
"We were impressed with the quality of the glyco-engineering system provided by Creative Biolabs for therapeutic glycoprotein development. They had a high level of professionalism and expertise. After understanding our needs, they developed a customized experimental protocol. In the end, the stability and activity of the glycoproteins were greatly improved, which exceeded our expectations. Their dedication to service quality and innovation was commendable."
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