Lectin Microarray

Services Published Data FAQs Customer Review

Lectin microarray is a useful approach for glycan profiling of the therapeutic proteins. With the years of experience in employing lectin microarray for glycan profiling, Creative Biolabs is confident to provide the most comprehensive glycan profiling service for our global clients with the highest quality and the most competitive price.

Technical Principle of Lectin Microarray

Lectin microarray is a sensitive and high-throughput technology for glycan profiling. Lectins are a group of glycan-binding proteins (GBPs) that can selectively interact with glycan molecules. In the design of lectin microarrays, the specified lectins are immobilized onto a chemically (e.g., epoxy, NHS ester) or biologically (e.g., streptavidin) activated solid phase. When a glycoprotein is incubated with the lectin chip, it will bind to the specific lectin and the binding events detected at specific lectin spots provide images of possible glycan variants in the sample. Lectin microarray has been widely used to assess glycan variants of purified glycoproteins and it is also an effective tool for glycan profiling of a therapeutic monoclonal antibody.

Fig.1 Construction steps of lectin microarray. Fig.1 The construction and application process of lectin microarray.^{1, 3}

Lectin Microarray for Glycoprotein Analysis Services in Creative Biolabs

As one of the leading companies in the glycoprotein analysis field, Creative Biolabs has over years of experience in glycosylation study. Based on the high throughput and sensitive lectin microarray platform, we can offer the comprehensive analysis services of Glycan Profiling for our customers. Besides, we also provide custom glycosylation analysis services based on lectin microarray assays, such as the rapid characterization of glycosylation in proteins, antibodies, hormones, and other glycoconjugates.

Advantages of Lectin Microarray for Glycoprotein Analysis

Compared to the MS-based approach, lectin microarray directly works on intact glycoproteins and has little effect on a testing sample (e.g., fluorescent labeling). Besides, when the lectin microarray approach is coupled with a sensitive detection system, it appears to offer high throughput and rapid screening of glycan profiling of therapeutic proteins.

Applications of Lectin Microarray

Rapid analysis of glycosylation profiles of glycoproteins and glycoform characterization.
Quantitative analysis of lectin-glycoprotein interactions.
Quality control of various glycoprotein products such as antibody drugs.
Profiling of bacterial cell-surface glycans and the mammalian cell-surface glycome.

Creative Biolabs has extensive experience in glycoprotein analysis. We have equipped a skilled expert team and a powerful technical platform, allowing us to provide high-quality glycoprotein analysis service. Based on lectin microarray, we can offer the most comprehensive glycan profiling and characterization of glycosylation in proteins. If you are interested in our glycoprotein analysis services using lectin microarray, please feel free to contact us for more details.

Published data

Many glycans on the cell surface can regulate the signaling molecules of stem cell growth and differentiation, thereby affecting the behavior of stem cells. As a high-throughput glycome analysis tool, lectin microarray can perform comprehensive glycome analysis of stem cells. In this study, the researchers isolated fresh epidermal stem cells from the skin of young and old mice. They conducted an in-depth glycome analysis of these stem cells using a microarray consisting of 96 lectins with different glycan binding properties. The results showed that there were significant differences in the glycan profiles of epidermal stem cells between young and old mice. With the advancement of the aging process, the glycosylation pattern of epidermal stem cells had changed significantly, specifically manifested as a decrease in mannose content and an increase in sialic acid modification. These glycan modifications can be used as molecular markers of aging in the future, helping us to better understand the mechanism of skin aging.

Fig.2 Epidermal stem cells-based lectin microarray analysis. Fig.2 Schematic diagram of lectin microarray of epidermal stem cells.^{2, 3}

FAQ

Q1: What types of biological samples can be analyzed by lectin microarray?

A1: Lectin microarray analyzes a variety of biological samples, including purified glycoproteins, monoclonal antibodies, cell culture supernatants, and other glycoconjugates. In addition, it is also suitable for complex samples such as serum and body fluids to evaluate the glycosylation characteristics and their relationship with biological functions.

Q2: Why choose lectin microarray instead of MS for glycosylation analysis?

A2: Compared with MS, lectin microarray directly analyzes intact glycoproteins with high throughput and high sensitivity without sample pretreatment, maintaining the integrity of the sample. In addition, lectin microarray has rapid screening capabilities and detection of multiple glycan specificities, which can simultaneously evaluate multiple glycosylation variants, making it more advantageous in large-scale screening and quantitative analysis.

Q3: How to choose the right lectin for specific glycan analysis?

A3: The selection of lectins should be determined according to the structure and composition of the target glycan. We provide relevant advice and recommend the most suitable lectin combination for your experimental design.

Customer Review

Efficient Analysis And Reliable Results
“I am very satisfied with Creative Biolabs' lectin microarray analysis service. The analysis process is fast, and I feel at ease with the reproducibility and reliability of the final results. In my research, we need a lot of glycosylation data, and this service helps us save a lot of time and obtain high-quality data.”

Flexible Customized Service
“I particularly appreciate Creative Biolabs' ability to provide customized lectin microarray analysis solutions according to our specific needs. Such flexibility allows us to better adjust the experimental design to suit different research goals. Very professional!”

Reference

Sun, Yangyang, et al. "A human lectin microarray for sperm surface glycosylation analysis." Molecular & Cellular Proteomics 15.9 (2016): 2839-2851.
Oinam, Lalhaba, et al. "Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging." Aging Cell 19.8 (2020): e13190.
Distributed under Open Access license CC BY 4.0, without modification.

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