Cellulase Activity Assessment Service

Professional Activity Assessment of Cellulase at Creative Biolabs

Cellulose is the main component of plant cell walls and is ubiquitous in many plants. Cellulase is a class of enzymes that efficiently break down cellulose into simpler carbohydrates, such as glucose. Cellulase includes several different types of enzymes, each showing specific activity under different conditions. For example, endoglucanase cleaves β-1,4-glucosidic bonds and is primarily responsible for cleavage within the cellulose chain. Exoglucanase starts from the end of the cellulose chain and gradually breaks down the cellulose into shorter cellulose or glucose units. β-Glucosidase is responsible for hydrolyzing the β-1,4-glucosidic bonds on the cellulose chain, releasing free glucose. These enzymes work together to make cellulase an effective cellulose decomposition tool and are widely applied in biofuel production, food processing, and many other fields.

Creative Biolabs has set up a laboratory specializing in enzyme activity analysis and biochemical testing. Our professional technical team provides various enzyme activity assessment services, such as cellulase, Cellulolytic Enzymes, and Amylolytic Enzymes. Activity analysis of cellulase can evaluate the efficiency and speed of cellulose degradation, help optimize the production process, improve cellulose degradation efficiency, save costs, and reduce waste. The activity analysis of cellulase usually includes the following content.

• Preparation of substrate solution

We prepare a reaction system containing cellulose substrate and use it as a matrix for the determination of cellulase activity.

• Addition of enzyme sample

We add the cellulase sample to be analyzed into the substrate solution and then start the reaction by setting appropriate conditions such as reaction temperature, pH value, and reaction time.

• Sampling

At different time intervals, we take some samples from the reaction solution and store them to analyze the cellulose degradation during the subsequent reaction.

• Cessation of reaction

When the reaction proceeds for a while, we stop the reaction by adding an acid or treating it at a high temperature.

• Product analysis

We use chemical methods (such as the catechol sulfate method) or biochemical analysis methods (such as the glucose oxidase reaction method) to measure the amount of glucose released in the reaction solution. In addition, chromatographic and fluorescence methods are also used to analyze the content of cellulose degradation products.

• Assessment of enzyme activity

We use glucose standards of known concentrations to establish a standard curve and calculate the glucose content in the experimental samples. Then according to the amount of glucose released and the reaction conditions, we can calculate the cellulase activity per unit time, which is usually expressed as the amount of glucose released per unit time (such as mg/min). Finally, we perform statistical analysis on the experimental data to evaluate the cellulase activity level under different samples or reaction conditions.

Creative Biolabs has rich experience and technical background in enzyme activity analysis and provides professional advice and custom enzyme activity assessment services according to client needs. In addition, we can also provide Biomass Enzyme Degradation Efficiency Analysis and Enzyme-Mediated Saccharification Efficiency Analysis services. if you would like to obtain reliable enzyme activity assessment services, please contact us in time, we are your best partner.

Published data

The authors developed a new method for cellulase and chitinase activity determination. They chose wild-type chitosan oligosaccharide oxidase ChitO to detect chitinase, and the ChitO-Q268R mutant to detect cellulase activity. These oxidases oxidized the hydrolysis products produced by chitinases and cellulases and released hydrogen peroxide. The authors then used other enzymes, such as horseradish peroxidase (HRP), to monitor hydrogen peroxide production, allowing for the measurement of chitinase and cellulase activities via a colorimetric method. The results showed that the activity detection of chitinase and cellulase was completed within 15 minutes. This method also detected individual chitooligosaccharides and cellooligosaccharides. Compared with traditional methods, ChitO-based determination does not require boiling or heating, has lower detection limits, simplified processing steps, and faster analysis efficiency. This newly developed enzyme activity assay method has significant application advantages in processes such as biorefining.

Fig.1 ChitO-Q268R mutant-based cellulase activity analysis results.¹

FAQs

Q1: What is cellulase activity assay? What is the principle of your analysis method?

A1: Cellulase activity assay is a process used to determine the efficiency of cellulases in degrading cellulose under specific conditions. The principle of our assay is to assess enzyme activity by measuring the concentration of reducing sugars produced by substrate degradation.

Q2: What types of samples are your analytical services suitable for?

A2: Our service can perform cellulase activity analysis on samples from different sources, including but not limited to microbial culture fluid, plant materials, etc.

Q3: How to prepare samples for cellulase activity analysis?

A3: Sample preparation methods usually include steps such as crushing, dissolving, or diluting to ensure that the cellulase in the sample can effectively react with the substrate.

Customer Review

Reference

1. Ferrari, Alessandro R., Yasser Gaber, and Marco W. Fraaije. "A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection." Biotechnology for Biofuels 7 (2014): 1-8.

Related Services:

1. Biomass Enzyme Degradation Efficiency Analysis
2. Enzymatic Decomposition Reaction-based Residue Content Analysis
3. Enzymatic Decomposition Reaction-based Fermentation Inhibitors Analysis
4. Enzyme-Mediated Saccharification Efficiency Analysis
5. Cellulolytic Enzyme Activity Assessment
6. Amylolytic Enzyme Activity Assessment