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Mass Spectrometry (MS)

Mass spectrometry (MS) is a powerful analytical technique that is widely used in the field of glycomics and glycobiology. Different separation methods such as capillary electrophoresis (CE) combined with MS is used to provide highly informative fragments for structural identification of glycoproteins. Based on MS technique, Creative Biolabs is able to offer the first-rate service of glycoprotein analysis for our customers.

Glycoproteins Analysis Based on MS

MS is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. It is widely used to detect and identify unknown compounds within a sample, quantify known materials, as well as to elucidate the structure and chemical properties of different molecules. An effective separation technique is required to increase sensitivity by reducing the number of species that endure simultaneous ionization, thus improving the high-quality characterization of the high complexity of glycan sample. Especially in glycoprotein analysis, MS in conjugation with different separation methods, such as CE, hydrophilic interaction chromatography (HILIC), and ion mobility, has emerged as a popular tool for glycoprotein analysis, providing highly informative fragments for structural identification of glycoproteins. For example, CE is a useful tool for glycan separation due to its high sensitivity and speed, as well as its isomer resolution and superior peak capacity. CE-MS is a useful tool in determining site-specific glycan micro-heterogeneity of glycoproteins at the glycopeptide level, as well as in peptide mapping and glycoform. HILIC provides better separation of hydrophilic glycopeptides as well as regular peptides but not glycopeptide isomers. ESI-MS and HILIC-MS are both used to determine the characterization of protein glycosylation site(s) and occupancy. Besides, ESI is also appropriate for the direct analysis of acidic glycans for MS and tandem MS. MALDI-MS is used extensively for analysis of glycan classes.

MS Analysis Services in Creative Biolabs

Professional experts in Creative Biolabs have worked on glycoproteins analysis research for many years. Hence, we are very confident to offer high-quality and comprehensive services of glycoproteins analysis for global clients. Our MS technology is usually combined with various separation techniques such as CE, ESI, HILIC, MALDI, making it a sensitive and rapid glycoproteins analysis platform. Based on these techniques, we offer a full range of glycoproteins analysis services including Glycan Profiling, Glycomic Profiling, Glycosylation Site Mapping, and Glycan Sequencing.

Features of Our Services

  • Experienced expert team
  • Advanced technique platforms
  • Providing accurate and comprehensive analysis data as quickly as possible
  • High quality analysis services

Equipped with diverse technologies and the best scientists, Creative Biolabs is capable of providing a full series of glycoprotein analysis services for clients all over the world. Based on MS technology, we are able to provide high-quality and low-cost services including glycan profiling, glycomic profiling and glycosylation site mapping. If you are interested in our MS analysis services, please feel free to contact us for more details.

Published data

Glycosylation modification plays a key role in proteins and participates in regulating physiological processes. Abnormal glycosylation leads to the occurrence of many diseases. Therefore, glycans have been widely applied in disease diagnosis and treatment monitoring. In this process, MS technology has become the preferred tool for studying glycan structure and quantitative analysis, which can distinguish glycan isomers and deepen the understanding of potential disease mechanisms. In this article, the author introduces the latest progress of MS-based glycoproteomics in the study of diseases such as pancreatic cancer and diabetes. In the MS analysis process, we first extract, enrich, and purify the target analytes from biological samples such as cells and tissues, then release glycosylated peptides by enzymatic digestion, and then use liquid chromatography-mass spectrometry technology for separation and analysis to determine the sugar chain structure, glycosylation type, and site. MS analysis usually breaks down large molecular glycosylated analytes into small fragments, which are suitable for identification by time-of-flight or Orbitrap mass spectrometers. Its specificity and high sensitivity make it a powerful tool for rapid and accurate measurement of biomolecules from various samples, accelerating the discovery and research process of biomarkers.

Fig.1 Glycoproteomic analysis process by MS.Fig.1 MS-based glycoproteomics analysis process.1

FAQs

Q1: What types of glycoprotein samples can be analyzed using MS at Creative Biolabs?

A1: At Creative Biolabs, our MS-based techniques analyze a wide range of glycoprotein samples, including those derived from human, animal, and plant sources. We cater to various sample types such as purified proteins, complex protein mixtures, and biological fluids. Our advanced instruments and diverse separation methods ensure reliable and accurate results regardless of the sample complexity.

Q2: What sample preparation steps are necessary for glycoprotein analysis using your MS services?

A2: Sample preparation for glycoprotein analysis typically involves several critical steps, including protein extraction, purification, and enzymatic digestion. Depending on the specific analysis, it might also involve glycan release, derivatization, or labeling. Our experts will tailor the preparation process to suit the specific needs of the analysis, ensuring to obtain optimal analysis results.

Q3: How do you handle data interpretation and reporting?

A3: Creative Biolabs provides detailed data interpretation and comprehensive reporting as part of our services. Our experienced scientists analyze the raw data, interpret the MS spectra, and provide a thorough report that includes all findings, relevant glycan structures, site-specific glycan micro-heterogeneity, and any additional insights. Clients will receive a clear, user-friendly report that facilitates further research or decision-making processes.

Customer Review

Excellent Analysis Technology Platform
"Creative Biolabs' MS analysis service is a truly efficient technology platform. I needed to conduct an in-depth structural analysis of a complex glycoprotein, and they provided detailed analysis data in a very short time, which not only saved my time but also ensured the accuracy and reliability of the experiment."

Diverse MS Analysis Technology
"Creative Biolabs' expert team demonstrated their deep expertise and rich experience. We submitted different types of glycoprotein samples, and they used a variety of advanced MS analysis technologies, such as HILIC-MS, and MALDI-MS, to ensure the accuracy and comprehensiveness of our glycoprotein analysis."

Reference

  1. Tabang, Dylan Nicholas, Megan Ford, and Lingjun Li. "Recent advances in mass spectrometry-based glycomic and glycoproteomic studies of pancreatic diseases." Frontiers in chemistry 9 (2021): 707387. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.

Related Services:

  1. Lectin Microarray
  2. LC-ESI-MS
  3. MALDI-TOF MS
  4. HPLC
  5. HPAEC-PAD
  6. RP-HPLC
  7. UHPLC/FLD/Q-TOF
  8. FTIR
  9. NMR
  10. SPRi
  11. TLC
  12. Flow Cytometry
  13. GC-MS
  14. Imaging Mass Spectrometry
  15. Glycobiology Microarray
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