Immuno Cell Glycoengineering Services

Comprehensive Immune Cell Glycoengineering Service at Creative Biolabs

Immune cells are a type of cells in the body that are responsible for immune defense functions. They can identify, attack, and eliminate pathogens (such as bacteria, and viruses) and abnormal cells (such as cancer cells) inside and outside the body, thereby maintaining the stability of the body's internal environment and resisting external aggression. The main immune cell types include T cells, NK cells, macrophages, and B cells. Carry out glycoengineering services on immune cells, which can change and optimize the glycosyl structure of immune cells, thereby enhancing their activity, recognition ability, and effector functions, and improving the attack power of immune cells against pathogens or abnormal cells.

Creative Biolabs has many years of experience in the field of Cell Line Glycoengineering. Our professional scientific research team masters the latest glycosyl engineering technology and methods, which can achieve precise glycosyl modification of immune cells and provide clients with a full range of immune cell glycoengineering services. We usually perform specialized glycoengineering services on immune cells through the following steps.

• Design of the scheme

We design corresponding glycoengineering schemes according to the client's needs, select the types of immune cells that require glycosyl modification, and determine the method of glycosyl modification.

• Cell culture and collection

We culture and expand immune cells, such as T cells, NK cells, or other immune cells, under appropriate laboratory conditions to ensure that sufficient cell numbers are obtained for subsequent processing.

• Glycosyl modification
  • NK Cell Glycoengineering Service
    We provide clients with high-level NK cell glycoengineering services. On the one hand, we perform metabolic glycoengineering on NK cells by changing the sugars or metabolic substances in the culture medium. On the other hand, we use click chemistry technology to directly combine click chemical reagents with receptors on the surface of NK cells to achieve stable glycosyl modification.
  • T Cell Glycoengineering Service
    We provide T cell glycoengineering services through a variety of technologies, including genetic engineering, chemical modification, viral vector-mediated glycosylation, and other technologies, to provide precise glycoengineering services for T cells.

• Glycosyl analysis

We use a variety of techniques to analyze glycans in glycoengineered cells, such as mass spectrometry to analyze and identify glycan structures and flow cytometry to detect changes in glycans on the cell surface.

• Functional assessment

We conduct in vitro verification and evaluation of glycosyl-modified immune cells, detect whether the changes in the glycosyl structure are as expected, and evaluate the functional changes of immune cells after glycosyl modification, including cell recognition ability, killing effect, etc.

Creative Biolabs leads the trend in the field of cell glycoengineering and is committed to providing clients with high-quality immune cell glycoengineering services. We provide personalized glycoengineering service according to client needs and flexibly respond to different research goals. Moreover, we utilize glycoengineered immune cells as a production platform to produce therapeutic proteins with specific glycosyl modifications that improve their stability, activity, and bioavailability. Please contact us if you need to obtain more information.

Published data

NK cells serve as immune cells and can kill tumor cytotoxic cells. The authors developed a glycoengineering strategy to modify NK cells to give them the targeting ability to recognize B-cell lymphoma. CD22 is a selective target for B-cell lymphoma, the authors successfully introduced 9-O-modified sialic acid-based CD22 ligands onto the surface of NK cells through metabolic glycoengineering and tested their activity in vitro and in mouse tumor models. The results showed that glycoengineered NK cells had a significant ability to bind and kill CD22⁺ lymphoma cells. This metabolic glycoengineering method is effective and convenient, which is a new anti-cancer immunotherapy strategy.

Fig.1 Metabolic glycoengineering strategies for NK cells.¹

FAQs

Q1: What specific technologies and methods are used for glycoengineering of immune cells? What are the advantages of these technologies?

A1: We usually use CRISPR-Cas9 technology, viral vector-mediated transfection, and chemical modification to perform glycoengineering of immune cells. CRISPR-Cas9 technology is highly precise and effective and can achieve targeted editing; viral vector-mediated transfection can efficiently introduce genetic information into target cells; chemical modification methods are flexible and can be used to introduce various sugar structures.

Q2: Can you help us design personalized immune cell glycoengineering protocols that meet specific research needs?

A2: Yes, our team customizes the immune cell glycoengineering program for you according to your specific research goals and needs to ensure that your research requirements are met and the expected results are achieved.

Q3: What are the costs of immune cell glycoengineering services? How long does it usually take to complete a project?

A3: Cost and project lead time will vary according to the complexity and requirements of the specific project. We provide you with a detailed quote and project schedule so that you know the cost and execution time of the service.

Customer Review

Reference

1. Wang, Xianwu, et al. "Glycoengineering of natural killer cells with CD22 ligands for enhanced anticancer immunotherapy." ACS Central Science 6.3 (2020): 382-389.

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