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Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

MALDI-TOF MS is one of the most popular techniques for glycan analysis due to its excellent speed, low sample consumption, soft ionization, high throughput, and automation potential. Based on this method, Creative Biolabs is able to offer a wide range of glycoprotein analysis services for customers all over the world with high quality and affordable cost.

Glycoproteins Analysis Based on MALDI-TOF MS

MALDI-TOF MS is a mass spectrometry technique that has been applied for many years in biopharmaceutical glycoanalysis. The new generation MALDI mass spectrometers can accurately determine the mass of proteins, peptides, and glycans, and segment these substances to obtain protein or polypeptide sequence data. MALDI is a soft ionization method that is developed for the ionisation of relatively large polypeptides and proteins, but its application has been expanded to glycoproteins, oligonucleotides, and complex carbohydrates. One of the advantages of MALDI-TOF MS is that the process of soft ionization causes almost no fragmentation of the analytes, and the molecular ions of the analytes can be identified even in the mixture. Consequently, MALDI-TOF MS is the pre-eminent technique for screening complex mixtures of glycans from biological extracts, thereby revealing the types of glycans present. Furthermore, other main advantages of MALDI-TOF MS technique include its excellent speed, low sample consumption, high throughput, and automation potential. Due to its speed and low consumption of sample, MALDI-TOF MS has been applied for oligosaccharide sequencing via exoglycosidase digestion, providing the information about anomericity of glycosidic linkages. For native glycans, detailed structural information can be achieved based on MALDI-MS/MS technique.

MALDI-TOF MS Analysis Services in Creative Biolabs

With the years of experience in glycoprotein analysis, Creative Biolabs has established a sensitive and high-throughput MALDI-TOF MS technique that is applied for analysis of types of glycans and structure of glycans. Based on MALDI-TOF MS technique, complex mixtures of glycans can be screened and separated from biological sample. This technology can also be used for oligosaccharide sequencing via exoglycosidase digestion and provide the information about anomericity of glycosidic linkages and identify the types of glycans. Due to its rapid speed, low sample consumption, and high throughput, MALDI-TOF MS has become a popular technique for glycans analysis.

Features of Our Services

  • Excellent technical knowledge
  • Sensitive and high-flux technique platforms
  • High quality analysis service
  • Comprehensive technical guidance
  • Accurate and comprehensive analysis data

Creative Biolabs is a world-leading service provider for glycoprotein analysis. Our professional team has engaged in the research of glycoprotein analysis for many years. We are very confident in offering a wide range of glycoprotein analysis services for global customers. Our analysis service items include Glycan Profiling, Glycomic Profiling, Glycosylation site mapping, and Glycan Sequencing. Besides, we provide custom service to meet all the clients’ special requirements. If you are interested in our MALDI-TOF MS analysis services, please don’t hesitate to contact us for more details.

Published data

Glycans affect the functions and application of proteins, and glycosylation is an important process of post-translational modification of cellular proteins. The analysis and quantification of protein glycosylation requires a combination of different techniques, such as enzyme reactions, isotope labeling, and MS. In this study, the authors developed a quantitative glycopeptide analysis method by combining stable isotope labeling and MALDI-TOF MS, which is efficient and sensitive. First, the authors synthesized and evaluated heavy and light isotope reagents (benzoic acid-d5 N-succinimidyl ester (d-BzOSu) and benzoic acid-d0 N-succinimidyl ester (BzOSu)), and prepared human serum IgG (hIgG) and IgG1 glycopeptides. Subsequently, the stable isotopes were used to label each glycopeptide, and the labeled glycopeptides were enzymatically reacted using endoS and β-N-acetylglucosaminidase. The reacted samples were then analyzed by MALDI-TOF MS in positive ion mode. The experimental results showed that this stable isotope labeling-based strategy achieved high-sensitivity quantification of glycopeptides and was a general and simple glycoproteomics analysis method.

Fig.1 Stable isotope labeled glycopeptides-based MALDI TOF-MS analysis process.Fig.1 Stable isotope labeling-based MALDI TOF-MS analysis strategy.1

FAQs

Q1: What types of glycoconjugates can you analyze?

A1: We analyze a wide range of glycoconjugate types, including but not limited to N-glycans, O-glycans, glycans, and complex carbohydrates. MALDI-TOF MS technology enables in-depth analysis of glycoconjugates and obtains detailed structural information. We also provide oligosaccharide sequencing services, using exoglycosidase digestion to provide information on glycosidic bond connections.

Q2: How should samples be prepared for MALDI-TOF MS analysis?

A2: The specific requirements for sample preparation depend on the type and goal of the analysis. In general, we recommend that samples should be purified and suitable for mass spectrometry analysis. For glycoconjugate analysis, samples may require enzymatic digestion or derivatization. In addition, samples should have as little contamination as possible to ensure accurate results. We provide comprehensive technical guidance to help clients prepare samples.

Q3: How accurate and reliable are the results of MALDI-TOF MS?

A3: The new generation of MALDI mass spectrometers we use have high precision and reliability, and can accurately measure proteins, peptides, and glycoconjugates. Through optimized sample preparation and analysis processes, we provide accurate and comprehensive analytical data. Our technical team has rich experience and strictly follows standard operating procedures to ensure high reproducibility and reliability of results.

Customer Review

Fast And Accurate Glycan Analysis
"Creative Biolabs' MALDI-TOF MS analysis service was fast, consumed minimal samples, and provided highly accurate glycan analysis results. This allowed us to quickly obtain detailed information on protein and peptide glycosylation, providing reliable data support for our subsequent drug development."

Provide Comprehensive Glycan Structural Information
"Through MALDI-TOF MS techniques, we obtained detailed structural information of complex glycans. Creative Biolabs' service not only helped us to analyze the type of glycans but also provided the anomeric structure information of the glycosidic bond through exoglycosidase digestion, which helped our glycosylation research."

Reference

  1. Kurogochi, Masaki, and Junko Amano. "Relative quantitation of glycopeptides based on stable isotope labeling using MALDI-TOF MS." Molecules 19.7 (2014): 9944-9961. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.

Related Services:

  1. Lectin Microarray
  2. MS
  3. LC-ESI-MS
  4. HPLC
  5. HPAEC-PAD
  6. RP-HPLC
  7. UHPLC/FLD/Q-TOF
  8. FTIR
  9. NMR
  10. SPRi
  11. TLC
  12. Flow Cytometry
  13. GC-MS
  14. Imaging Mass Spectrometry
  15. Glycobiology Microarray
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