Terpene Quantitative Profiling Service

Reliable Terpene Analysis Service at Creative Biolabs

Creative Biolabs has strong comprehensive strength in terms of talent, equipment, experience, and management. We provide global clients with a full range of quantitative analyses for Polyamine, Polyphenol, and terpene in Biomass samples. Terpene has anti-inflammatory, antioxidant, antibacterial, and other activities, and in-depth analysis of different types of terpene in biomass helps identify new medicinal plant resources and provide new sources of materials for the food industry. We use gas chromatography-mass spectrometry (GC-MS) to quantitatively analyze terpene in biomass through the following steps.

• Sample preparation

We grind the biomass sample into powder, then use solvents such as ethyl acetate and n-hexane to extract terpene in the sample and concentrate it by rotary evaporation to obtain a concentrated terpene sample.

• Chromatographic separation

We use gas chromatography to separate various terpenoid compounds in the sample. We choose the HP-5MS capillary column with a good separation effect as the chromatographic column, set a low initial temperature of the chromatographic column (for example, 50°C), and then heat it to a higher temperature at a certain rate. The separation of terpene components in the sample is achieved by gradient elution.

• Mass spectrometry analysis

We use the internal standard method or external standard method to analyze the terpene components in biomass samples through a mass spectrometry detector. The internal standard method requires the addition of internal standard substances of known concentration to the sample, while the external standard method uses standard substances of known concentration to establish a standard curve. We identify various terpene components in the GC-MS chromatogram, such as pinene, linalool, limonene, β-myrcene, α-terpineol, etc. We compare the ratio of the peak area of terpene in the sample to be tested with the corresponding peak area in the internal standard or standard curve, calculate the content of terpene, and generate the final analysis report.

Creative Biolabs has a good industry reputation and client reputation, providing clients with professional and reliable terpene analysis services. In addition, we have strong data processing and analysis capabilities and provide clients with detailed analysis reports and feasible suggestions. If you need to analyze terpene in biomass samples, please contact us to obtain custom analysis services exclusively for you.

Published data

Terpenes are hydrocarbons that are classified according to the number of units or functional groups. Terpenes give biomass cannabis its unique odor and pharmacological properties. Currently, methods for quantifying terpenes in cannabis require large amounts of biomass samples, long extraction times, and long chromatographic elution times, which are not suitable for analysis in a high-throughput environment. Therefore, the authors developed a rapid GC-MS method to achieve high-throughput analysis of terpenes in biomass cannabis. They optimized the sampling technique, chromatographic columns, and solvents. The results showed that on and DB17 columns, the resolution of each sample peak was high, and all compounds could be eluted within a run time of 30 minutes. In addition, the peak shape of compounds extracted with hexane was good. Therefore, the authors used hexane to extract terpenes from cannabis and performed GC-MS analysis using dodecane as an internal standard. The results showed that a total of 48 terpenoids were detected in biomass cannabis. Moreover, the authors performed methodological validation for 22 of these terpenoids, including parameters such as detection limit, quantification limit, linearity, precision, and accuracy. The authors believe that this GC-MS analysis method is suitable for high-throughput analysis of biomass cannabis.

Fig.1 Effects of different extraction solvents on peak shape: isopropanol (red), ethyl acetate (green), hexane (black).^{1, 2}

FAQs

Q1: Can you provide detailed information about sample preparation?

A1: Of course, we can guide you on how to properly prepare samples, including grinding, extraction, and pretreatment steps to ensure the best analytical results.

Q2: What data analysis and result interpretation do you provide?

A2: We usually report the total content of terpenes, and provide analysis results of different terpene classes as required. We will provide a detailed data analysis report, including chromatograms, peak identification, content calculation method, and result interpretation.

Q3: Can you customize the analysis according to my needs?

A3: Yes, we provide custom analysis services according to the specific needs of clients, including the detection of specific terpene components, adjustment of quantitative range, etc. You can discuss customized needs further with our expert team.

Customer Review

References

1. Krill, Christian, Simone Rochfort, and German Spangenberg. "A high-throughput method for the comprehensive analysis of terpenes and terpenoids in medicinal cannabis biomass." Metabolites 10.7 (2020): 276.
2. Under Open Access license CC BY 4.0, without modification.

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