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As a leading specialist in glycoscience, Creative Biolabs is proud to launch our high-throughput glycan screening service to help our worldwide clients with their cutting-edge research projects. By applying our staffs’ experience and a proven quality system, our scientists employ two strategies, including ultran high-performance liquid chromatography with fluorescence detection(UHPLC-FLD) and fluorescence-labeled lectin-based glycan assay for high-throughput glycan screening. Moreover, we also offer high-quality high-throughput titer assays for antibody/Fc fusion protein and cell line development.
Glycoproteins play key roles in various molecular and cellular functions and are among the most difficult to analyze biomolecules on account of their microheterogeneity, low abundances, and non-template-driven synthesis. The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins are regulated by their glycosylation profile. Thus, there is increasing demand for the qualitative and quantitative characterization of glycosylation on glycoproteins in all biologics development stages. Creative Biolabs has developed two simple and efficient strategies for high-throughput glycan screening on antibodies, Fc fusion proteins, non-Fc fusion proteins, or in glycoengineered cell lines. Minimal samples can be used for high-throughput glycan screening in all biologics development stages. Our strategies can automate the high-throughput glycan screening on large sample sets of crude cell culture supernatants and purified intact glycoproteins.
Besides high-throughput glycan screening, scientists at Creative Biolabs have developed several efficient titer assays, including antibody/Fc fusion protein titer assay and high-throughput titer assay for cell line development.
Our antibody/Fc fusion protein titer assay enables the rapid titer analysis of antibodies/Fc fusion proteins in small volumes of cell culture supernatant. This assay can be analyzed automatically in 25 minutes in a 384-well plate without sample preparation.
Our automated high-throughput titer assay for cell line development has several advantages, including high speed, high accuracy, and low price.
With proven experience in high-throughput glycan screening and titer assay, Creative Biolabs is an ideal company to entrust with your business. Through our high-throughput system, our clients could accelerate their research with reduced project costs and timelines. For more detailed information, please feel free to contact us or directly send us an inquiry.
Glycan structure has a great influence on the biological functions of glycoproteins. Several high-throughput glycan analysis and screening techniques are available to analyze glycan profiles, such as UHPLC-FLD, etc. This study developed a robust and specific high-throughput method to purify and analyze the glycosylation pattern of human plasma transferrin. The researchers discovered a broader range of the structures through analysis, particularly in terms of sialic acid linkages for trisialylated and disialylated structures. This study not only provides the study of glycosylation patterns of human plasma transferrin with data support but also provides theoretical support for the development of high-throughput glycan screening techniques.
Fig.1 Isolation and N-glycan characterization of human plasma transferrin.1
Q1: What studies are high-throughput glycan screening often used for?
A1: Glycosylation not only affects the structure of therapeutic glycoproteins but also has an impact on their functions. High-throughput glycan screening is essential for the study of therapeutic glycoproteins and the rapid screening of specific glycoform glycoproteins. In addition, it is also used for biomarker discovery, biopharmaceutical development, and so on.
Q2: What are the advantages of using UHPLC-FLD for high-throughput glycan screening?
A2: UHPLC-FLD is a highly efficient method for glycan analysis with several advantages.
Extensive Experience in High-throughput Glycan Screening
"We felt very fortunate to have chosen Creative Biolabs' high-throughput glycan screening service. Their staff were very experienced in this field. After understanding our needs, they utilized the advanced technology they developed to help us quickly analyze the structure of the glycan and screen for specific cell lines. The fast lab cycle effectively accelerated our research process. This service was highly recommended."
Experts in High-throughput Glycan Screening
"Creative Biolabs impressed us with the quality and efficiency of their high-throughput glycan screening service. From sample handling to data analysis, they were in contact with us at all times to ensure that our needs were maximized. There was no doubt that they are experts in high-throughput glycan screening."
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