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High-throughput Glycan Screening Service

As a leading specialist in glycoscience, Creative Biolabs is proud to launch our high-throughput glycan screening service to help our worldwide clients with their cutting-edge research projects. By applying our staffs’ experience and a proven quality system, our scientists employ two strategies, including ultran high-performance liquid chromatography with fluorescence detection(UHPLC-FLD) and fluorescence-labeled lectin-based glycan assay for high-throughput glycan screening. Moreover, we also offer high-quality high-throughput titer assays for antibody/Fc fusion protein and cell line development.

High-throughput Glycan Screening Service at Creative Biolabs

Glycoproteins play key roles in various molecular and cellular functions and are among the most difficult to analyze biomolecules on account of their microheterogeneity, low abundances, and non-template-driven synthesis. The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins are regulated by their glycosylation profile. Thus, there is increasing demand for the qualitative and quantitative characterization of glycosylation on glycoproteins in all biologics development stages. Creative Biolabs has developed two simple and efficient strategies for high-throughput glycan screening on antibodies, Fc fusion proteins, non-Fc fusion proteins, or in glycoengineered cell lines. Minimal samples can be used for high-throughput glycan screening in all biologics development stages. Our strategies can automate the high-throughput glycan screening on large sample sets of crude cell culture supernatants and purified intact glycoproteins.

  • High-throughput glycan screening by using UHPLC-FLD
    • Samples are analyzed by UHPLC-FLD. As short as 1 week for up to 100 samples.
    • Samples as crude as clarified cell culture media can be processed. Our seasoned scientists offer comprehensive analysis of various time points and conditions to support our clients’ cell culture development program's needs.

  • High-throughput glycan screening by glycan assay
    • Fluorescence-labeled lectin-based glycan assay.
    • Detection of different types of important glycans, including high mannose, fucose, galactose, and sialic acid.
    • Screening on antibodies, Fc fusion proteins, non-Fc fusion proteins, or in glycoengineered cell lines.
    • The whole process takes roughly 2 hours.

Features of Our High-throughput Glycan Screening Service Including but not Limited to:

  • Fluorescence labeled lectins to detect different types of glycosylation
  • High mannose, fucose, galactose, and sialic acid identification
  • Discrimination of 2,3 and 2,6 N-sialylation patterns
  • Both crude cell culture supernatants and intact glycoproteins are available

High-throughput Glycan Screening Service 2

Applications of High-throughput Glycan Screening Including but not Limited to:

  • Media optimization
  • Glycan monitoring during bioreactor runs
  • Accelerating target clone screening
  • Glycoengineered cell line screening

Titer Assays

Besides high-throughput glycan screening, scientists at Creative Biolabs have developed several efficient titer assays, including antibody/Fc fusion protein titer assay and high-throughput titer assay for cell line development.

  • Antibody/Fc fusion protein titer assays

    Our antibody/Fc fusion protein titer assay enables the rapid titer analysis of antibodies/Fc fusion proteins in small volumes of cell culture supernatant. This assay can be analyzed automatically in 25 minutes in a 384-well plate without sample preparation.

  • High-throughput titer assay for cell line development

    Our automated high-throughput titer assay for cell line development has several advantages, including high speed, high accuracy, and low price.

With proven experience in high-throughput glycan screening and titer assay, Creative Biolabs is an ideal company to entrust with your business. Through our high-throughput system, our clients could accelerate their research with reduced project costs and timelines. For more detailed information, please feel free to contact us or directly send us an inquiry.

Published data

Glycan structure has a great influence on the biological functions of glycoproteins. Several high-throughput glycan analysis and screening techniques are available to analyze glycan profiles, such as UHPLC-FLD, etc. This study developed a robust and specific high-throughput method to purify and analyze the glycosylation pattern of human plasma transferrin. The researchers discovered a broader range of the structures through analysis, particularly in terms of sialic acid linkages for trisialylated and disialylated structures. This study not only provides the study of glycosylation patterns of human plasma transferrin with data support but also provides theoretical support for the development of high-throughput glycan screening techniques.

Fig.1 Processes for isolation and N-glycan characterization.Fig.1 Isolation and N-glycan characterization of human plasma transferrin.1

FAQs

Q1: What studies are high-throughput glycan screening often used for?

A1: Glycosylation not only affects the structure of therapeutic glycoproteins but also has an impact on their functions. High-throughput glycan screening is essential for the study of therapeutic glycoproteins and the rapid screening of specific glycoform glycoproteins. In addition, it is also used for biomarker discovery, biopharmaceutical development, and so on.

Q2: What are the advantages of using UHPLC-FLD for high-throughput glycan screening?

A2: UHPLC-FLD is a highly efficient method for glycan analysis with several advantages.

  • High sensitivity: UHPLC-FLD detects glycans at very low concentrations, improving the sensitivity of glycan analysis.
  • High resolution: UHPLC-FLD has a higher resolution for accurate analysis of glycans.
  • High throughput and speed: UHPLC-FLD analyzes multiple samples in a short period, making it ideal for high-throughput glycan screening applications.

Customer Review

Extensive Experience in High-throughput Glycan Screening
"We felt very fortunate to have chosen Creative Biolabs' high-throughput glycan screening service. Their staff were very experienced in this field. After understanding our needs, they utilized the advanced technology they developed to help us quickly analyze the structure of the glycan and screen for specific cell lines. The fast lab cycle effectively accelerated our research process. This service was highly recommended."

Experts in High-throughput Glycan Screening
"Creative Biolabs impressed us with the quality and efficiency of their high-throughput glycan screening service. From sample handling to data analysis, they were in contact with us at all times to ensure that our needs were maximized. There was no doubt that they are experts in high-throughput glycan screening."

Reference

  1. Trbojević-Akmačić, Irena, et al. "Comparative analysis of transferrin and IgG N-glycosylation in two human populations." Communications biology 6.1 (2023): 312. Distributed under Open Access license CC BY 4.0, without modification.
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