Glycosylation Analysis Service for MERS-CoV Glycoprotein
MERS-CoV Glycoprotein
The Middle East respiratory syndrome coronavirus (MERS-CoV) is one of the coronaviruses previously considered relatively harmless respiratory pathogens in humans. MERS-CoV features spike (S) glycoprotein on its surface, specifically a type I membrane glycoprotein consisting of 1353 amino acids, which plays a pivotal role in facilitating the virus's entry into host cells by interacting with the cellular receptor dipeptidyl peptidase 4 (DPP4). The S protein of MERS-CoV undergoes cleavage in the process of infection, resulting in the formation of a receptor-binding subunit S1 and a membrane-fusion subunit S2.
Glycosylation of MERS-CoV S protein primarily involves N-linked glycans, these glycans are typically complex in structure and can vary in composition. Glycosylation influences the interaction between the S protein and host cell receptors, potentially affecting the virus's ability to attach to and enter host cells. The glycans on viral proteins can act as a shield, masking the underlying viral proteins from recognition by the host immune system. Understanding the glycosylation of MERS-CoV is essential for gaining insights into viral biology, pathogenesis, and potential targets for therapeutic interventions.
Fig.1 Structure and genome composition of MERS-CoV.1, 2
MERS-CoV-2 Glycosylation Analysis Services at Creative Biolabs
Analyzing the glycosylation of the S protein is crucial for understanding its role in virus-host interactions and for developing strategies to combat MERS-CoV infection. To facilitate this, Creative Biolabs has developed glycosylation analysis services specifically tailored to characterize the glycosylation patterns of the MERS-CoV S glycoprotein. Our comprehensive glycosylation analysis services include:
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Glycosylation site mapping: We identify and map glycosylation sites on the MERS-CoV S glycoprotein.
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Comprehensive glycan analysis: We perform in-depth analysis of glycans at each site, including composition, structure, and abundance assessments.
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High-resolution mass spectrometry: We utilize high-resolution and high-accuracy mass spectrometry techniques to ensure precise and reliable results. We utilize LC-MS/MS for data acquisition and employ the combination of electron transfer dissociation (ETD) and high collision dissociation (HCD) fragmentation capabilities, enhancing the analysis of glycopeptides.
Our team follows stringent protocols in sample handling and analysis to ensure the best quality results. We are dedicated to delivering cutting-edge glycosylation analysis for the MERS-CoV S glycoprotein.
Highlights
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Specialized procedures for glycan analysis
We offer specialized Standard Operating Procedures (SOPs). Our team is dedicated to maintaining the highest standards for sample handling and analysis.
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Latest Equipment for MS Analysis
We utilize cutting-edge equipment, including Orbitrap Fusion (CID/HCD/ETD) and QTOF high-resolution MS platforms, guaranteeing comprehensive and precise glycan analysis.
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Professional team for glycosylation analysis
Our laboratory is staffed with highly experienced experts who specialize in every aspect of the glycosylation analysis workflow, from sample preparation to MS data acquisition and interpretation.
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High service quality with a short turnaround time
Creative Biolabs is committed to delivering high-quality glycosylation analysis services, providing you with top-quality and timely results to meet your research needs.
Understanding the glycosylation patterns associated with the S protein is of paramount importance. Analyzing the glycosylation of the S protein aids in deciphering its role in the virus-host interaction and guides efforts aimed at combating MERS-CoV infection. Creative Biolabs has developed versatile platforms to conduct viral glycosylation analysis for MERS-CoV. Our specialized SOPs, expert team, and state-of-the-art equipment make us a trusted partner for glycosylation analysis. Please don't hesitate to contact us for more information or to submit an inquiry directly. Our expertise in glycosylation analysis can contribute significantly to your research.
References
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Xu, Jiuyang, et al. "Antibodies and vaccines against Middle East respiratory syndrome coronavirus." Emerging microbes & infections 8.1 (2019): 841-856.
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Under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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