Plant Vitamins Content Profiling Service

Comprehensive Vitamins Content Analysis Service in Plant at Creative Biolabs

Various vitamins, including fat-soluble and water-soluble vitamins, occur naturally in plants. Analysis of plant vitamins can help evaluate the nutritional value of plant foods, understand the content and types of vitamins in them, and guide people to develop a balanced meal plan to promote a healthy lifestyle. Moreover, plant vitamin analysis helps control product quality and optimizes production processes, thereby improving food safety, and production efficiency. Creative Biolabs has an experienced and professional scientific research team. We conduct comprehensive and in-depth analyses of plant vitamins and provide custom analysis plans and solutions for different plant sample types.

• Sample pretreatment

We grind plant biomass samples into fine powder or undergo appropriate pretreatment to make the vitamins in the samples easy to extract.

• Extraction of vitamins

• Mixing: We mix the sample with a fat-soluble solvent (such as ether, chloroform, etc.) to extract fat-soluble vitamins. Or mix the sample with water or aqueous solvents (such as methanol, ethanol, etc.) to extract water-soluble vitamins.
• Extraction: We extract fat-soluble and water-soluble vitamins through ultrasonic-assisted extraction (UAE), supercritical fluid extraction (SFE), solid-phase extraction (SPE), liquid-liquid extraction (LLE), etc.
• Separation and purification: We separate the extract and solid residue through centrifugation and other methods, and use a solid-phase extraction column to remove impurities in the supernatant.
• Concentration: Then we use an evaporator or nitrogen purge to concentrate the extract to obtain concentrates of fat-soluble and water-soluble vitamins respectively.

• Analysis of vitamins

• High-performance liquid chromatography (HPLC): We use a liquid chromatography column to separate the sample, and then quantify the vitamins through a UV detector or fluorescence detector.
• Liquid chromatography-mass spectrometry (LC-MS): This detection method is highly sensitive and accurate, and can analyze multiple fat-soluble and water-soluble vitamins simultaneously.
• Capillary electrophoresis (CE): This method has a good separation effect and fast analysis speed, and is suitable for the analysis of water-soluble vitamins such as vitamin B and vitamin C.

Our powerful Plant Chemistry Profiling platform provides clients with multiple types of high-quality vitamin analysis services, including but not limited to the following types:

• Fat-soluble vitamins: Vitamin A, vitamin D2, vitamin D3, vitamin E acetate, vitamin K1;
• Water-soluble vitamins: Vitamin B (such as Vitamin B1, B2, B3, B5, B6, B9, B12), vitamin C;

Through these analysis steps, Creative Biolabs accurately determines the vitamin content in plant biomass, providing important data support for nutritional assessment, product quality control, and scientific research. Additionally, we also provide Pigment Profiling, Cell Wall Analysis, and Elemental Analysis. Please contact us if you need a specific analysis quote.

Published data

Tetrapleura tetraptera is a tropical leguminous plant whose fruits are rich in various nutrients and have anticonvulsant, antibacterial, and other medicinal activities. The author selected T. tetraptera as the research object and studied the levels of various vitamins and trace elements in its seeds, pulp, and whole fruit to gain an in-depth understanding of its nutritional composition and trace element extraction rate. The authors first ground the obtained seeds and pulp into a fine powder and mixed them to form a whole fruit sample. Then different extraction methods were used to extract water-soluble vitamin C, fat-soluble vitamins A, E, and β-carotene, and the vitamin content in each sample was quantitatively analyzed by spectrophotometry, titration, and HPLC. For trace elements, the authors analyzed the elements in the sample using a flame atomic absorption spectrometer after nitric acid digestion. The table below lists the vitamin contents of different sample parts. The results showed that there were significant differences in the vitamin C and E contents of the pulp, seeds, and whole fruits of T. tetraptera, while there were no significant differences in vitamin A and β-carotene. This study provided important advice on which parts of T. tetraptera to choose for consumption.

Table 1 Analysis of vitamin components in different parts of T. tetraptera.¹

FAQs

Q1: Is the vitamin analysis method you used suitable for my specific plant sample?

A1: Our vitamin analysis methods apply to various types of plant samples. In addition, we will adjust the analysis method according to the characteristics of your sample to ensure accurate measurement of the results.

Q2: Can you test the stability of vitamins?

A2: Yes, we evaluate the stability of vitamins by analyzing samples under different storage conditions and provide recommendations on optimal storage.

Q3: How much does a vitamin content test cost?

A3: Our prices depend on the sample quantity, complexity, and scope of analysis required. If you have analytical needs, please contact us for a detailed quote to fit your budget.

Customer Review

Reference

1. Oteng, Prince, et al. "Vitamin analysis, trace elements content, and their extractabilities in Tetrapleura tetraptera." Journal of Chemistry 2020 (2020).

Related Services:

1. Plant Pigment Profiling
2. Plant Cell Wall Composition Analysis
3. Plant Microelement and Macroelement Analysis