Apiose Analysis Service

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Various Analysis methods for Apiose at Creative Biolabs

Apiose is a unique branched-chain pentose sugar that exists in plant cell walls and secondary metabolites. It has high biological activity, and it does not exist in free form but mainly exists in the structure of apigenin and pectin. It has many applications in the pharmacology and medical aesthetics industries. There is therefore a need to develop reliable analytical methods for the detection of apiose in various raw materials and related chemical products. Creative Biolabs has established a mature Carbohydrate Analysis platform, and our professional technical team provides various Monosaccharide, Oligosaccharide, and Polysaccharide analysis services. Apiose is a monosaccharide and we have developed efficient methods to selectively quantify apiose.

High-Performance Liquid Chromatography (HPLC)

We place the sample to be tested in trifluoroacetic acid or sulfuric acid solution and hydrolyze the sample through continuous stirring and heating. The hydrolysis solution is then neutralized with barium carbonate, and the solution is centrifuged and filtered to obtain the solution to be analyzed. We then use ligand exchange chromatography (HPLEC) to separate apiose and other monosaccharides, derivatize it with L-arginine after the column, and combine it with fluorescence detection technology to achieve quantitative determination of apiose. This method is highly sensitive and easy to operate, which is used for the analysis of apiose in a variety of plant samples and liquid samples.

Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS)

We have developed a HILIC-MS/MS method to separate and detect common monosaccharides and apiose. HILIC is suitable for the separation of polar and hydrophilic compounds and effectively separates polar polysaccharide compounds such as apiose. In addition, tandem MS technology improves the sensitivity of detection and achieves highly selective analysis and quantitative determination of apiose in complex sample matrices.

Gas Chromatography-Mass Spectrometry (GC-MS)

We treat plant polysaccharides with anhydrous methanol containing HCl to release their neutral and acidic monosaccharides, including apiose. The samples are then further acetylated or silylated to form monosaccharide derivatives, and finally, various monosaccharide derivatives are identified and quantitatively analyzed by GC-MS. Each glycosyl residue is identified by its multimodal characteristic pattern, retention time, and specific fragment ions in the mass spectrum.

Ion Exchange Chromatography (IE)

In addition, IE can also effectively separate and analyze apiose and has good selectivity when other ionic impurities are present in the sample. We have combined IE with pulsed amperometric detection (PAD) to achieve the separation and analysis of common monosaccharides and apiose.

Professional apiose analysis services. (Creative Biolabs Original)

Creative Biolabs has a powerful analytical technology platform. We provide high-quality monosaccharide analysis services such as apiose, Xylulose, Xylonic Acid, etc. Please feel free to contact us if you would like to obtain more analysis details.

Published data

Duckweed is an aquatic plant that can accumulate rich carbohydrates in its leaves through photosynthesis. In this study, the authors cultured five different genera of duckweed under two light intensities to explore the relationship between their relative growth rate (RGR) and carbohydrate content, thereby evaluating the effect of growth rate on carbohydrate metabolism. The results showed that when the light intensity was high, the amount of starch accumulated by the faster-growing Lemnoideae species was lower than that of the slower-growing Wolffioideae species. In addition, high light intensity also promoted the growth of the Wolffioideae species and increased the proportion of apiose in its cell walls. The authors believed that apiose levels were an effective marker for assessing duckweed growth ability, and boron absorption might be an important factor controlling the growth of this aquatic plant.

Fig.1 Apiose content in duckweed cell walls under two light intensities. Fig.1 Analysis of apiose in duckweed cell walls under different light intensities.¹

FAQ

Q1: Is your laboratory experienced with apiose analysis?

A1: Yes, our laboratory has extensive experience in analyzing apiose and uses advanced analytical technologies and methods to ensure accuracy and reliability.

Q2: How long does the analysis process for apiose take?

A2: The analysis time of apiose depends on the number and complexity of the sample. Usually after receiving the sample, we will start the analysis as soon as possible and provide the results within the agreed time.

Q3: What types of samples can you analyze for apiose?

A3: We provide apiose analysis for various sample types, including in plant tissues, food, chemical products, etc. If you have special requirements, please let us know so we make arrangements accordingly.

Customer Review

Customized Analysis Service
"Creative Biolabs was able to accommodate our specific apiose analysis requirements and provided customized solutions tailored to our needs. Very flexible and accommodating!"

Professional Analysis Team
"I was impressed by the professionalism and accuracy of the apiose analysis service provided by Creative Biolabs. The results were detailed and reliable, helping us greatly in our research."

Reference

Pagliuso, Débora, et al. "Correlation of apiose levels and growth rates in duckweeds." Frontiers in Chemistry 6 (2018): 291. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only.