Xylonic Acid Analysis Service

Comprehensive Analysis of Xylonic Acid at Creative Biolabs

Xylose, an abundant carbohydrate, exists in nature and can be converted into a variety of substances through oxidation-reduction reactions, such as xylonic acid, xylitol, lactic acid, etc. Xylonic acid is a biodegradable compound that is used as a biobased chemical to replace traditional chemicals. Moreover, it has important application value in biopharmaceuticals, the food industry, and other fields. Xylonic acid is used as an intermediate in the production of certain types of antibiotics and other drugs and is also used as a food additive. Therefore, analysis for xylonic acid can ensure product quality and safety, optimize production processes, and provide key information and support for related applications in various fields. Creative Biolabs has established a mature Carbohydrate Analysis platform, and our strong technical team provides clients with a wide range of sugar analysis services, including Monosaccharides, Oligosaccharides, and Polysaccharides. Xylonic acid is a monosaccharide for which we provide comprehensive analysis services.

• Sample preparation

We collect different samples, such as fermentation broth, culture medium, and other samples containing xylonic acid, and perform filtration, dilution, and other processing on them. For solid samples, we need to extract xylonic acid from the sample and purify the extracted solution to remove interfering substances.

• Prepare standard curve

We prepare a series of standard solutions of xylonic acid whose concentration range should cover the concentration of the compounds to be tested. A standard curve is then established by high-performance liquid chromatography (HPLC).

• Sample separation

We select a C18 column as the chromatographic column, set appropriate mobile phase and gradient conditions, and then inject the extracted and purified samples and standard solutions into the HPLC so that xylonic acid appears at the appropriate time point and is well separated.

• Sample testing

We detect xylonic acid through the diode array detector DAD. After determining the retention time of xylonic acid, we compare it with a standard to determine the presence of xylonic acid.

• Data processing and analysis

We perform data processing on HPLC spectra, including peak area integration, peak identification, etc. Then, according to the drawn standard curve, the concentration of xylonic acid in the sample is calculated using the peak area comparison or peak height comparison method. In addition, if necessary, we can use mass spectrometry (MS) analysis technology for structural identification and further confirmation of xylonic acid.

Creative Biolabs has extensive experience in carbohydrate analysis and can accurately and reliably perform xylonic acid analysis and interpret the results. We have strict quality control and quality assurance systems to ensure that the analysis process meets standards and provides high-quality analysis reports. In addition, we also provide analysis for special monosaccharides such as Apiose and Xylulose. If you need high-level sugar analysis services, please contact us in time and we will respond to your needs quickly.

Published data

The efficiency of lignocellulose metabolism and xylose fermentation is low. In order to effectively reduce commercial costs, xylose needs to be converted into high-value chemicals. For example, xylose is converted into xylonic acid. Xylonic acid is a water-soluble organic acid that is non-volatile, non-toxic, and non-corrosive. However, xylonic acid faces the challenge of low productivity during the fermentation process. Therefore, in order to improve the bioconversion efficiency of xylonic acid, in this study, the authors optimized three key process fermentation parameters to increase the yield and conversion efficiency of xylonic acid. They used high-performance anion exchange chromatography combined with pulsed amperometric detection to determine the content of xylonic acid under different process conditions and determined the optimal fermentation process parameters. These results were of great significance and application value for improving the production efficiency of xylonic acid.

Fig.1 Xylonic acid (XA) yield under optimized conditions.¹

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Reference

1. He, Tao, et al. "Optimization of specific productivity for xylonic acid production by gluconobacter oxydans using response surface methodology." Frontiers in Bioengineering and Biotechnology 9 (2021): 729988. Distributed under Open Access license CC BY 4.0, without modification.

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• Apiose Analysis
• Xylulose Analysis