Xylulose Analysis Service
Reliable Xylulose Analysis at Creative Biolabs
Xylulose is a natural five-carbon sugar that exists in bacteria, fungi, and plants, and is an inducer of ATP catabolism. Changes in xylulose levels may be related to certain diseases or pathophysiological conditions, such as diabetes, metabolic diseases, etc. Xylulose analysis is therefore useful for disease diagnosis and treatment monitoring. Moreover, xylulose, as a metabolite, has potential use in the field of drug development. Analysis of its content and metabolic pathways can help evaluate the effectiveness and safety of drugs.
As an expert in the field of Carbohydrate Analysis, Creative Biolabs provides clients with professional Monosaccharide, Oligosaccharide, and Polysaccharide Analysis services. Among them, xylulose is a specialty sugar, and we usually analyze it through the following steps.
We collect the samples to be analyzed and process them appropriately, including extraction, purification, dilution, and other steps to ensure that the xylulose content in the samples can be accurately determined.
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Establishment of the standard curve
We prepare a series of xylulose standard solutions with known concentrations to construct a standard curve for the subsequent determination of xylulose content in unknown samples.
We use a variety of analytical techniques to measure xylulose in the sample and calculate the xylulose content in the sample by comparing the peak areas of the sample and the standard curve. Common analysis methods include:
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Enzymatic method: We add the sample to the reaction system containing xylulokinase. Xylulokinase can catalyze the reaction between xylulose and ATP to generate xylulose-5-phosphate. Then we measure the absorbance change of the reaction product with a photometer and calculate the concentration of xylulose in the sample. This method is used for rapid analysis of xylulose content. It is easy to perform in the laboratory and has certain accuracy and sensitivity.
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High-performance liquid chromatography (HPLC): We inject the processed sample into an HPLC and use the distribution coefficients of different components between the mobile phase and the stationary phase to separate xylulose from other compounds.
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Mass spectrometry (MS): We ionize the sample in a mass spectrometer, and then perform quantitative or qualitative analysis of xylulose according to the mass spectrum of the ion mass-to-charge ratio. This method is widely used for the analysis of xylulose in various complex mixtures and has the characteristics of high selectivity and high sensitivity.
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Data processing and analysis
We perform data processing and analysis on the measurement results to ensure the accuracy and reliability of the results.
Through the above steps, Creative Biolabs provides clients with accurate and reliable xylulose analysis services to help them deeply understand the role and significance of xylulose in different biological systems. In addition, we also provide other specialty sugars analysis services, including Apiose and Xylonic Acid Analysis services. Please contact us if you would like to get detailed analysis details.
Published data
The analysis of sugars is important in many fields, providing critical information for product development, research, and quality control. For large numbers of samples that need to be analyzed quickly, colorimetric methods according to absorbance or fluorescence measurements are more advantageous. Furanose, especially d-xylulose, d-fructose, and 1-deoxy-d-xylulose-5-phosphate (DXP) is commonly measured by the Kulka method. It is the oxidation of furanose by ferric ammonium sulfate to generate a furfural product and then react with resorcinol to generate a stable colored product. Finally, the target compound is quantified by measuring the absorption spectrum of the product.
Fig.1 Absorption spectrum of the products of the resorcinol-furanose reaction; (A) d-fructose, (B) d-xylulose and DXP.1, 2
FAQs
Q1: How long does it take to complete a xylulose analysis?
A1: Usually the time to analyze a sample depends on the number of samples and the experimental load. We will process your sample as quickly as possible and provide an accurate analysis report after the experiment. Depending on the sample quantity, it usually takes a few days to a week.
Q2: What quality control steps did you take in your experiments to ensure data accuracy?
A2: We implement strict quality control procedures, including repeated measurements, verification using standard curves, quality inspection samples, etc., to ensure the reliability and accuracy of data.
Q3: If I need further analysis or other services, do you provide custom services?
A3: Yes, we provide custom analysis services according to your needs. Please tell us your needs and we are happy to serve you.
Customer Review
Accuracy
"Thank you very much to your team for providing us with accurate and timely xylulose analysis services. Your experimental reports are very clear and the data are accurate and reliable. We are satisfied with the results."
Service Quality
"I highly recommend Creative Biolabs's xylulose analysis service! Your team is efficient and professional, and the results are reliable. It's worth working with."
References
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Shaw, Shreya, and Robin Ghosh. "A modified Kulka micromethod for the rapid and safe analysis of fructose and 1-deoxy-D-xylulose-5-phosphate." Metabolites 8.4 (2018): 77.
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Under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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