α-Glucan and β-Glucan Content Analysis Service

Accurate α-Glucan and β-Glucan Content Analysis Service in Biomass Process Liquid at Creative Biolabs

Biomass naturally contains polysaccharides. Some plant biomass, such as wood, pulp, straw, etc., will release polysaccharides (such as α-glucan and β-glucan) after biomass conversion or other processing, and convert them into soluble forms, causing them to appear in the treatment fluid. α-Glucan is a polymer composed of α-glucose units, commonly found in starch. β-Glucan is a polymer composed of β-glucose units commonly found in cellulose walls. The existence of these polysaccharides mainly originates from the composition of the biomass itself, as well as the conversion and generation during biomass processing. Creative Biolabs has established a comprehensive Biomass Profiling Platform. We provide diversified analysis services such as Lignocellulose Profiling, Process Liquid Analysis, and Seaweed Profiling. Analysis of α-glucan and β-glucan in biomass process liquid helps optimize biomass conversion processes, monitor the quality of biomass processing processes, and provide critical information and support to all aspects of the biomass processing industry.

• Step 1: Sample preparation

We process samples obtained from biomass process liquid through filtration, dilution, etc.

• Step 2: Determination of total sugar content
  • Preparing a standard curve: We prepare a series of glucose standard solutions of known concentrations and develop a standard curve.
  • Determination of total sugar content: We mix the sample with phenol reagent, measure the absorbance after adding sulfuric acid, and calculate the total sugar content according to the standard curve.
• Step 3: Determination of α-glucan and β-glucan content
  • Enzymatic hydrolysis: We use appropriate enzymes (such as α-amylase or β-glucosidase) to hydrolyze samples into monosaccharides or oligosaccharides. The conditions for enzymatic hydrolysis (time, temperature, pH, etc.) need to be optimized according to experimental requirements.
  • Determination of α-glucan and β-glucan: We perform quantitative analysis of α-glucan and β-glucan using suitable analytical methods such as chromatography (HPLC) or mass spectrometry (MS).
  • In addition, we measure the absorbance of the colored product generated by the reaction through the iodine reaction method and the methyl ketone reagent method to analyze the α-glucan and β-glucan content in the sample.
• Step 4: Data analysis

We accurately record the experimental process and result data, calculate the α-glucan and β-glucan contents in biomass process liquid according to the above-mentioned measurement methods, and provide a final report or conclusion for our clients.

Fig.1 Steps of α-glucan and β-glucan content analysis.

Creative Biolabs masters the analysis methods and technologies of α-glucan and β-glucan in biomass process liquid. We provide clients with comprehensive and professional α-glucan and β-glucan content analysis services in biomass process liquids to help clients understand the composition of their biomass liquids. In addition, we have efficient sample processing capabilities and a rapid response mechanism, which can complete analysis and provide reports promptly. If you need to analyze various types of Sugars or Degradation Products in your biomass process liquid samples, please contact us and we will provide you with the best analysis services.

Published data

Walnut green shells are agricultural waste generated when walnuts are harvested. These green shells are rich in vitamins and phenolic compounds and are one of the potential sources of high-value compounds. In this study, the authors selected walnut green shells obtained under different soil conditions (Montalto Uffugo and Zumpano) as the research object, and the collected shells were pre-treated by freeze drying and grinding. Then, they used acidic and alkaline extraction methods to separate pectin and glucan from dry walnut shell powder and measured crude glucan by spectroscopy, morphology, and thermodynamics。 Moreover, enzymatic determination of α- and β-glucan was performed by colorimetry. The results showed that the total glucan yield extracted from walnut shells in the Montalto area was the highest, and the proportion of β-glucan was higher than that in Zumpano. This showed that walnut green shells could be used as a potential source of glucan and pectin, but their content and thermal properties would be affected by different soil conditions.

Fig.2 Strategies for the recovery of glucans and pectins from walnut green shells.¹

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Reference

1. La Torre, Chiara, et al. "Green husk of walnuts (Juglans regia L.) from southern Italy as a valuable source for the recovery of glucans and pectins." Fermentation 7.4 (2021): 305. Distributed under Open Access license CC BY 4.0, without modification.

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