α-Glucan and β-Glucan Content Analysis Service

Accurate α-Glucan and β-Glucan Content Analysis Service in Biomass Process Liquid at Creative Biolabs

Biomass naturally contains polysaccharides. Some plant biomass, such as wood, pulp, straw, etc., will release polysaccharides (such as α-glucan and β-glucan) after biomass conversion or other processing, and convert them into soluble forms, causing them to appear in the treatment fluid. α-Glucan is a polymer composed of α-glucose units, commonly found in starch. β-Glucan is a polymer composed of β-glucose units commonly found in cellulose walls. The existence of these polysaccharides mainly originates from the composition of the biomass itself, as well as the conversion and generation during biomass processing. Creative Biolabs has established a comprehensive Biomass Profiling Platform. We provide diversified analysis services such as Lignocellulose Profiling, Process Liquid Analysis, and Seaweed Profiling. Analysis of α-glucan and β-glucan in biomass process liquid helps optimize biomass conversion processes, monitor the quality of biomass processing processes, and provide critical information and support to all aspects of the biomass processing industry.

• Step 1: Sample preparation

We process samples obtained from biomass process liquid through filtration, dilution, etc.

• Step 2: Determination of total sugar content
  • Preparing a standard curve: We prepare a series of glucose standard solutions of known concentrations and develop a standard curve.
  • Determination of total sugar content: We mix the sample with phenol reagent, measure the absorbance after adding sulfuric acid, and calculate the total sugar content according to the standard curve.
• Step 3: Determination of α-glucan and β-glucan content
  • Enzymatic hydrolysis: We use appropriate enzymes (such as α-amylase or β-glucosidase) to hydrolyze samples into monosaccharides or oligosaccharides. The conditions for enzymatic hydrolysis (time, temperature, pH, etc.) need to be optimized according to experimental requirements.
  • Determination of α-glucan and β-glucan: We perform quantitative analysis of α-glucan and β-glucan using suitable analytical methods such as chromatography (HPLC) or mass spectrometry (MS).
  • In addition, we measure the absorbance of the colored product generated by the reaction through the iodine reaction method and the methyl ketone reagent method to analyze the α-glucan and β-glucan content in the sample.
• Step 4: Data analysis

We accurately record the experimental process and result data, calculate the α-glucan and β-glucan contents in biomass process liquid according to the above-mentioned measurement methods, and provide a final report or conclusion for our clients.

Fig.1 Steps of α-glucan and β-glucan content analysis.

Creative Biolabs masters the analysis methods and technologies of α-glucan and β-glucan in biomass process liquid. We provide clients with comprehensive and professional α-glucan and β-glucan content analysis services in biomass process liquids to help clients understand the composition of their biomass liquids. In addition, we have efficient sample processing capabilities and a rapid response mechanism, which can complete analysis and provide reports promptly. If you need to analyze various types of Sugars or Degradation Products in your biomass process liquid samples, please contact us and we will provide you with the best analysis services.

Published data

β-glucan, one of the most abundant polysaccharides, is present in the cell wall of the mushroom fruiting body and has multiple pharmacological effects and low toxicity. This polysaccharide is widely used in the food, health care, pharmaceutical, and cosmetic industries. The authors conducted a comparative analysis of β-glucan composition in two types of Enoki mushrooms (wild type and commercial type) of the same species under the same substrate and environmental conditions. They validated the results of the enzymatic β-glucan assay kit using HPLC. Moreover, the authors used atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) to characterize and analyze its structure. The results showed that the β-glucan content levels in the extracts of the two types of Enoki mushrooms were similar, but the commercial type of β-glucan was more soluble. The author used HPLC to quantify the total glucan in Enoki mushrooms, and the results were close to those determined by the enzyme kit. FT-IR and NMR results showed that the two types of Enoki mushrooms had similar β-glycosidic bond structures. However, the α-configuration has only been confirmed in commercial Enoki mushrooms. AFM results showed that the β-glucan of commercial Enoki mushrooms has a shorter, narrower, and multi-branched spatial structure. These findings illustrated the value of commercial Enoki mushroom β-glucan for applications in food or healthcare fields.

Fig.2 Analysis and characterization of β-glucan in two types of Enoki mushrooms.¹

FAQs

Q1: How to ensure the reliability of analysis results?

A1: Our analytical procedures are rigorously validated and follow standard operating procedures to ensure accuracy. All experiments are conducted under the guidance of trained professionals.

Q2: How does analyzing α-glucan and β-glucan affect my biomass processing process? How stable are these components during biomass processing?

A2: By analyzing the α-glucan and β-glucan content in biomass process liquids, we help you evaluate and optimize the biomass processing process, thereby improving production efficiency and product quality. Additionally, we provide information on the stability of α-glucan and β-glucan during biomass processing to help you optimize your process.

Q3: Do you offer custom analysis services to meet specific needs?

A3: Yes, we provide custom analysis services according to client needs to ensure specific requirements and goals are met.

Customer Review

Reference

1. Rezaeian, Sharareh, Hamid R. Pourianfar, and Samaneh Attaran Dowom. "Comparative assessment of β-glucan composition and structural characterization in wild and commercial Enoki mushrooms, Flammulina velutipes." Journal of Food Composition and Analysis 120 (2023): 105286.

Related Services:

1. Simple Sugar & Oligosaccharide Quantitative Profiling
2. Furan and Organic Acid Analysis
3. Trimer and Dimer Analysis
4. Maltooligosaccharide Quantitative Analysis
5. Degree of Esterification Determination
6. Degree of Acetylation Determination
7. Chitin Profiling