Amino Acid Quantitative Profiling Service

Discovering the Mysteries of Biomass with Amino Acid Analysis

The amino acid analysis provides researchers with detailed information about the composition and concentration of amino acids in a sample. This enables accurate and detailed characterization of the amino acid content of Biomass samples such as Lignocellulose, Seaweed, etc., to support product development, material valuation, and scientific research. Creative Biolabs provides a one-stop amino acid analysis service to support our client's research needs for quality assessment, compositional analysis, etc.

Accurate amino acid analysis is essential for biomass such as lignocellulose transformation, compositional analysis, and other various studies. Our optimized amino acid analysis method is very efficient and gives accurate results. The method detects 17 amino acids in lignocellulose and other biomass.

• Type of amino acid tested
  • Arginine: As a polar amino acid, only L-arginine has biological activities, including inhibition of inflammatory responses, activation of antioxidant responses, etc.
  • Alanine: As a neutral amino acid, it has two conformations (D-type and L-type) and is widely distributed in all living organisms. Biomasses such as lignocellulose are rich sources of free alanine.
  • Glycine: As a non-polar α-amino acid, glycine has a variety of uses, such as the manufacture of herbicides, surfactants, etc.
  • Aspartic acid: As an acidic alpha-amino acid, it has two conformations: D-type and L-type. Among them, D-aspartic acid is an amino acid of physiological significance.
  • Cysteine: As a polar amino acid, it is a sulfur-containing amino acid. L-cysteine is used in food and cosmetics.
  • Glutamic acid: As one of the constituents of protein, it is a polar and acidic alpha-amino acid.
  • Histidine: It is a polar essential amino acid with an imidazole functional group and is used as a feed supplement and skin conditioner. Soy protein is a rich source.
  • Lysine: As a polar alpha-amino acid, it has a positively charged primary amine group and is the limiting amino acid in grains.
  • Isoleucine: It is a non-polar and branched-chain alpha-amino acid that plays a vital role in protein metabolism, glucose consumption, and so on.
  • Leucine: As a non-polar alpha-amino acid, it has an isobutyl side chain and plays a role in protein synthesis.
  • Serine: It is a polar non-essential alpha-amino acid with a hydroxyl group that plays a role in many cellular processes.
  • Methionine: It is a sulfur-containing non-polar essential amino acid. In addition to its involvement in metabolism, it is used in the synthesis of a variety of biological compounds.
  • Tyrosine: It is an aromatic amino acid with a hydroxyl group that plays a crucial role in the biosynthesis of many important compounds.
  • Phenylalanine: As a non-polar alpha-amino acid with a benzyl group, it is a precursor substance for tyrosine synthesis.
  • Valine: As a non-polar alpha-amino acid, it is one of the essential amino acids in the human body. Valine is found to have a variety of physiological functions, including regulation of metabolic processes and plant growth.
  • Proline: It is a non-polar amino acid whose exogenous application protects plants from abiotic stress-related damage.
  • Threonine: It is a polar alpha-amino acid with a hydroxyl side chain that plays an important role in glycine biosynthesis.
• Steps for measuring the content of various amino acids
  • Protein hydrolysis
    We first treat the sample by acid hydrolysis to break down the proteins into individual amino acids. No acid treatment is required for the detection of free amino acids.
  • Removal of impurities
    The sample treated by acid hydrolysis may contain other non-amino acid compounds. We purify them using an ion exchange column.
  • Derivatization
    We perform derivatization to increase the detection sensitivity of some amino acids on analytical instruments.
  • Separation and quantification
    Separation and quantitative analysis are performed for each amino acid.
  • Results processing
    We determine the content of each amino acid in the sample and analyze the experimental results.

Fig.1 Process of amino acid analysis.

Advantages of Amino Acid Analysis Service

• Experienced: We provide an amino acid analysis for individual lignocellulosic and other biomass samples according to our technical strengths and rich analytical experience.
• Accurate results: Our optimized amino acid analysis method ensures better separation and detection of many types of amino acids, resulting in accurate analysis results.
• Wide range of services: Our amino acid analysis service applies to a wide range of lignocellulosic, and other biomass materials.

Analyzing amino acids in biomass provides valuable information about the protein composition of a sample. Creative Biolabs provides a professional amino acid analysis service to researchers, biomass processors, and others. In addition to lignocellulose, we also offer a Seaweed Amino Acid Analysis Service. We have a professional team to assist in the design of all amino acid analysis experiments. Welcome to contact us to learn more about amino acid analysis.

Published data

Amino acid content analysis is an effective method for biomass protein quality assessment. Microbial biomass as a protein source has been widely studied for its nutritional value as a food substitute. In the present study, protein digestibility and carbohydrate fermentation capacity of different microbial biomass samples (bacteria, fungi, yeast) in the colon were analyzed by amino acid analysis, and their nutritional quality was assessed. Ultimately it was found through the study that these biomass samples exhibited good protein digestibility. Meanwhile, heat treatment reduced their protein digestibility. This study provided effective support to analyze the amino acid content and nutritional value of these microbial biomass samples. Also through this study, we found that amino acid analysis had a vital role in biomass application research.

Fig.2 Protein digestibility of individual microbial biomass samples in the gastric and intestinal phase.^{1, 2}

FAQs

Q1: What is the difference between free amino acids and hydrolyzed amino acids?

A1: Free amino acids mean that the amino acids in the sample exist directly in the free state and can be measured without hydrolysis. Hydrolyzed amino acid means that the sample contains proteins, peptides, and other components, which need to be hydrolyzed into individual amino acids by hydrolysis and other pre-treatment before measurement.

Q2: Why do we measure the 17 amino acids in the sample?

A2: Three amino acids (tryptophan, glutamine, and asparagine) in proteins are readily lost or converted during acid hydrolysis. For example, tryptophan is easily degraded under acid conditions. Glutamine and asparagine are converted to glutamic acid and aspartic acid, respectively. Therefore, although proteins consist of 20 standard amino acids, we usually only test for 17 amino acids.

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References

1. Emilia, Nordlund, et al. "In vitro protein digestion and carbohydrate colon fermentation of microbial biomass samples from bacterial, filamentous fungus and yeast sources." Food Research International (2024): 114146.
2. Under Open Access license CC BY 4.0, without modification.

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