Protein Calibration Service

Trust Creative Biolabs for Protein-Corrected Klason Lignin Analysis Solutions

Lignin is a complex class of organic polymers found in various biomasses such as lignocellulose. Corrected lignin content analysis helps to better evaluate the potential of various Lignocellulosic and other Biomass feedstocks for bioenergy production and other industrial applications. Creative Biolabs provides a one-stop protein-corrected Klason lignin analysis service to facilitate lignocellulosic composition analysis and quality assessment for our clients.

We analyze protein-corrected Klason lignin in various lignocellulosic and other biomasses. As with ash analysis, starch content analysis, uronic acid analysis, etc., we have optimized and validated our protein-corrected Klason lignin analysis method to ensure the accuracy and precision of our results.

• Steps for protein-corrected Klassen lignin analysis

The content of Klason lignin is calculated by the difference between the mass of the acid-insoluble residue and the mass of the treated residue. Impurities such as proteins and nitrogen compounds remain in the acid-insoluble residue, which affects the detection of lignin content. Therefore, we also perform a test for the content of protein in the acid-insoluble residue and correct the lignin content according to protein contamination. In addition to this, during the analysis of Klason lignin, we also measure the content of nitrogen, hydrogen, carbon, and sulfur in the acid-insoluble residue using an elemental analyzer.

• Acid hydrolysis treatment of the sample
  Before testing the lignin content, the sample first needs to be treated with acid hydrolysis. The treatment process may vary for different sample types.
• Determination of content
  The acid-insoluble residue is obtained by drying. We detect and calculate the content of nitrogen, carbon, hydrogen, sulfur, protein, and lignin contained in the acid-insoluble residue.

• Delivered experimental results

• Klason lignin (protein corrected) content
• The protein content of acid-insoluble residue
• The hydrogen, carbon, sulfur, and nitrogen content of acid-insoluble residue

Fig.1 Procedure for analysis of protein-corrected Klason lignin.

Features of Protein-Corrected Klason Lignin Analysis Service

• Professional analysts: Our analysts are experienced and develop custom analysis plans according to client needs and sample types.
• Guaranteed experimental cycle: The analytical methods we use have been optimized and are very efficient. After clarifying the client's needs, we carry out the analysis and deliver the results as quickly as possible.
• Accurate results: Samples are analyzed in duplicate to ensure accurate and precise results.

Creative Biolabs is a leader in lignocellulose analysis. We develop individualized protocols according to the needs of our clients and perform careful and precise sample handling and protein-corrected Klason lignin analysis. Please feel free to contact us for more information and a detailed quote on protein-corrected Klason lignin analysis. We are always at your disposal for any assistance.

Published data

Lignocellulose is often used as animal feed. Therefore detection of lignin content in it is used to quantify its nutritional value as animal feed. In order to investigate an efficient and accurate method for lignin detection, this study compared the lignin content of forages using different analytical methods and evaluated the accuracy of these methods. By comparing these methods, the researchers found that protein affected the lignin content detected by the gravimetric method. Therefore the researchers suggested correcting for proteins to more accurately express lignin content. In the end, this study showed us how to efficiently and accurately detect the lignin content in lignocellulose, which provided us with a basis and data support for optimizing lignin detection methods.

Fig.2 Correlation analysis of lignin obtained by solubilization with sulfuric acid and corrected for protein.¹

FAQs

Q1: Why is a protein correction required for the detection of Klason lignin?

A1: The content of Klason lignin is calculated from the difference between the acid-insoluble residue and the residual mass after subsequent treatment. Acid-insoluble residues contain proteins that can interfere with the lignin content to a certain extent. Therefore, a protein correction for lignin content is required to obtain a more accurate lignin content during the testing process to accurately assess the value of lignocellulose.

Q2: Is it possible to pre-treat the samples?

A2: Our lab can pre-treat samples by grinding, etc. This may increase the experimental period to some extent. After the client explains the type of sample and specific requirements, we will develop an exclusive analysis plan according to the actual situation to maximize the satisfaction of the client's requirements.

Customer Review

Reference

1. Gomes, Daiany I., et al. "Evaluation of lignin contents in tropical forages using different analytical methods and their correlations with degradation of insoluble fiber." Animal Feed Science and Technology 168.3-4 (2011): 206-222.

Related Services:

1. Non-combustible Substance Content Analysis
2. Extractive Profiling
3. Carbohydrate Compositions Quantitative Profiling
4. Lignocellulose Lignin Profiling
5. Amylopectin and Amylose Profiling
6. Simple Sugars Qualitative and Quantitative Profiling
7. Acetyl Group Analysis
8. Amino Acid Quantitative Profiling
9. Fatty Acid Quantitative Profiling
10. Lignin Monomers Profiling