Anti-A. castellanii cysts Monoclonal Antibody (Mouse mAb)

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Key Details
Catalog MAS-0124-YJ6
Applications FC; IHC
Host Mouse
Reactivity A. castellanii
Clonality Monoclonal
Conjugations Conjugation could be customized
Sub CAT. Applications Clone Conjugations Endotoxin Level Size Quantity
MAS-0124-YJ6-A FC, IHC NFD1
Successfully!
MAS-0124-YJ6-B FC, IHC NFD2
Successfully!
MAS-0124-YJ6-C FC, IHC UBD1
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MAS-0124-YJ6-D FC, IHC UBD2
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Overview
Properties
Target

Overview

Description NFD1, NFD2, UBD1 and UBD2 are mouse IgG antibodies that recognize A. castellanii. These mAbs bind to AC112 cysts and do not bind to trophozoites. NFD1, NFD2 and UBD1 have cross-reactivity of Acanthamoeba castellanii (AC021), Acanthamoeba polyphaga (AC019), Acanthamoeba polyphaga (AC010), Acanthamoeba lenticulata (AC230) (NFD2 weak), Acanthamoeba culbertsoni (AC001). UBD2 does not bind to Acanthamoeba lenticulata (AC230) and weakly bind to Acanthamoeba polyphaga (AC019) and Acanthamoeba polyphaga (AC010). They do not bind to Acanthamoeba tubiashi (AC064).
Reactivity A. castellanii
Applications FC; IHC
Host Mouse
Immunogen NFD1, NFD2: Acanthamoeba castellanii strain 112 (AC112) Live trophozoites, cysts;
UBD1: Sonicated trophozoites;
UBD2: Formalin-fixed trophozoites.
Isotype NFD1, NFD2: IgG2a kappa
UBD1, UBD2: IgG1 kappa
Clonality Monoclonal
Clone NFD1; NFD2; UBD1; UBD2

Properties

Expression Host HEK293F/CHO
Conjugations Conjugation could be customized
Purification Protein A/G affinity purified is the regular method
Purity SDS-PAGE> 95%
Endotoxin Level Endotoxin level could be customized
Form Liquid (frequently-used) or lyophilized
Concentration About 1mg/ml. There are differences between batches. Special concentration could be customized.
Sterility 0.2 μM filtered
Buffer See the Datasheet
Storage See the Datasheet

Target

Target A. castellanii cysts
Alternative Names Acanthamoeba castellanii; A. castellanii; cysts; Acanthamoeba
Introduction A. castellanii can be found at high densities in various soil ecosystems. It preys on bacteria, but also fungi and other protozoa. This species is able to lyse bacteria and produce a wide range of enzymes, such as cellulases or chitinases, and probably contributes to the breakdown of organic matter in soil, contributing to the microbial loop.
Organism Protozoa
Related Disease Keratitis and granulomatous amoebic encephalitis (GAE)
Infections Route Open wound
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