Creative Biolabs offers anti-microbiome Ab secondary antibodies which can be used to amplify signals in immunoanalyses, such as Western blot, immunohistochemistry and immunocytochemistry, immunoprecipitation, ELISA, and fluorescent in situ hybridization.
Introduction of Anti-Microbiome Ab Secondary Antibodies
Rabbits, sheep, goats, mice, and donkeys were selected hosts for secondary antibodies. Secondary antibodies require different primary antibodies. They must be specific to both the type and isotype of the primary antibody used to indirectly detect the target to which a particular primary antibody first binds. The formation of anti-microbial antibodies is particularly driven by persistent bacterial translocation. Anti-microbial antibodies may be viable medium-term biomarkers for future clinical applications, including the prediction of the severity of cirrhosis and bacterial complications. It has been suggested that increased anti-microbial Abs are associated with dysfunctions of the intestinal barrier and an exaggerated immune response to luminal antigens. The presence of anti-microbial antibodies is not specific for some diseases, but the mechanism behind their formation may be specific, however, primarily associated with different diseases. Many researchers have focused on the ability of microorganisms to shape the immune system by stimulating B-cell and antibody responses and functional differentiation of T-helper cells.
Fig.1 Overview of tyramide signal amplification technique. (Kuczkiewicz, 2022)
Secondary Antibody Applications
The secondary antibody has two functions, one is to amplify any signal, and the other is easy to detect. Secondary antibodies are used for all types of immunoassays, most commonly Western blot, immunohistochemistry, and immunocytochemistry, and occasionally for immunoprecipitation, ELISA, and fluorescence in situ hybridization. ELISA and flow cytometry is used to detect secondary antibodies in basic studies, clinical analysis, and disease diagnosis.
Related Anti-Microbiome Antibody Products
If you are interested in our anti-microbiome Ab secondary antibodies products, please contact us for more details.
Reference
- Kuczkiewicz, S.O.; et al. The Role of Pathology-Based Methods in Qualitative and Quantitative Approaches to Cancer Immunotherapy. Cancers. 2022, 14(15): 3833.