Creative Biolabs has extensive experience to offer antibody development services for clients. Depending on novel research methods and tools. We are capable of meeting the specific need of each customer with the best project design.
Anti-Microbiome Monoclonal Antibody Development
Antibody development is the process to identify novel antibodies against different pathogens and diseases. The complete antibody drug development process is mainly divided into five stages, including target identification and validation, hit identification, hit to lead, lead optimization, and IND-enabling. Equipped with world-leading technology platforms and professional scientific staff, now we can provide a series of sources for anti-microbiome antibody discovery and development.
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Hybridoma Technology
Hybridoma technology is one of the most commonly used methods for monoclonal antibody production. After the antigen is obtained, the mice are immunized, and the antibody-producing B lymphocytes are then isolated and fused with an immortalized myeloma cell line to form hybrid cells. These hybridoma cells have the ability to produce a certain monoclonal antibody against the antigen.
Fig.1 Activation of B cells and production of antibodies. (Mitra, 2021)
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Surface Display
As an alternative to traditional hybridoma technology, the surface display is a recombinant technology that expresses a protein of interest on the cell surface. The obtained antibody genes are randomly cloned for surface display. Now we can provide phage display service, bacterial display service, ribosome display service, yeast display service, and mammalian cell display service. Antibodies with specific binding affinity for a particular antigen are then obtained through a selection and affinity enrichment process known as biopanning.
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B Cell Sorting
B cell sorting is the novel platform that enables rapid acquisition of fully human monoclonal antibodies or natural monoclonal antibodies from other species through high-throughput screening. In this process, B cells isolated from human peripheral blood or the spleen of immunized animals are subjected to deep sequencing and single B cell analysis techniques to obtain antibody candidates.
Recombinant Anti-Microbiome Antibody Production
Recombinant antibodies refer to antibody fragments produced based on recombinant antibody coding genes. Compared with conventional antibodies, recombinant antibodies present a series of advantages, such as being easy to engineer, wider biodistribution, no animal immunization required, and avoiding potentially deleterious Fc effector functions. With our highly trained and experienced technicians, we can offer both mammalian cell expression systems and prokaryotic expression systems, including HEK293 cells, CHO cells, E. coli cells, and yeast cells.
Creative Biolabs is a leading service provider that focuses on antibody discovery and production, we are confident in offering the best and most suitable products for our customers. If you are interested in our products, please do not hesitate to contact us for more details.
- Mitra, S.; Tomar, P.C. Hybridoma technology; advancements, clinical significance, and future aspects. Journal of Genetic Engineering and Biotechnology. 2021, 19(1): 1-12.