Key Details
| Catalog | MAS-0524-YJ3 |
| Applications | WB; FC; IP; ELISA; Neut; Inhib |
| Host | Human |
| Reactivity | C. difficile |
| Clonality | Monoclonal |
| Conjugations | Conjugation could be customized |
Overview
Properties
Target
Overview
| Description | Clone rB-B4, B-F5, OB41, BE90 are human IgGs recognizes C. difficile TcdB. rB-B4 and B-F5 shows strong binding activity in ELISA and exhibits the excellent neutralizing activity in Caco-2 and Vero cells. OB41 recognizes a single, highly conserved epitope on the TcdB glucosyltransferase domain and blocks productive translocation and delivery of the enzymatic cargo into the host cell. BE90 can neutralize C. difficile effects. |
| Reactivity | C. difficile |
| Applications | WB; FC; IP; ELISA; Neut; Inhib |
| Host | Human |
| Epitope |
rB-B4; B-F5; BE90: CROP domain OB41: GTD |
| Isotype |
rB-B4; B-F5; BE90: Human IgG1 kappa OB41: Humanized IgG1 |
| Clonality | Monoclonal |
| Clone | rB-B4; B-F5; OB41; BE90 |
| Dilutions |
ELISA: 0.1-1000 ng/mL WB: 0.2-1 μg/mL |
Properties
| Expression Host | HEK293F/CHO |
| Conjugations | Conjugation could be customized |
| Purification | Protein A/G affinity purified is the regular method |
| Purity | SDS-PAGE> 95% |
| Endotoxin Level | Endotoxin level could be customized |
| Form | Liquid (frequently-used) or lyophilized |
| Concentration | About 1mg/ml. There are differences between batches. Special concentration could be customized. |
| Sterility | 0.2 μM filtered |
| Buffer | See the Datasheet |
| Storage | See the Datasheet |
Target
| Target | C. difficile TcdB |
| Alternative Names | Clostridioides difficile Toxin B; C. difficile Toxin B; Toxin B; C. difficile; Clostridioides difficile; TcdB |
| Introduction | Two homologous exotoxins, toxin A (TcdA, molecular weight: 308 kDa) and toxin B (TcdB, molecular weight: 270 kDa), are major virulence factors of C. difficile, which contain four functional domains, each playing a role in a multi-step mechanism of entry. |
| Organism | Bacteria |
| Related Disease | Clostridium difficile infection and C. difficile-associated disease |
| Infections Route | Oral cavity, skin, alimentary canal |
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