Key Details
| Catalog | MAS-0524-YJ77 |
| Applications | ELISA |
| Host | Human |
| Reactivity | Cockroach |
| Clonality | Monoclonal |
| Conjugations | Conjugation could be customized |
| Sub CAT. | Applications | Clone | Conjugations | Endotoxin Level | Size | Quantity | |
|---|---|---|---|---|---|---|---|
| MAS-0524-YJ77-A | ELISA | h7C11 |
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Overview
Properties
Target
Overview
| Description | Clone 7C11 is a Mouse monoclonal IgG recognizes Cockroach Bla g 2. |
| Reactivity | Cockroach |
| Applications | ELISA |
| Host | Human |
| Epitope | The most important residues were K132 for the mAb 7C11 epitope and I199 and K251 for the mAb 4C3 epitope. |
| Isotype | Chimeric (mouse/human) IgG1 KAPPA |
| Clonality | Monoclonal |
| Clone | h7C11 |
| Dilutions | ELISA: 0.1-1000 ng/mL |
Properties
| Expression Host | HEK293F/CHO |
| Conjugations | Conjugation could be customized |
| Purification | Protein A/G affinity purified is the regular method |
| Purity | SDS-PAGE> 95% |
| Endotoxin Level | Endotoxin level could be customized |
| Form | Liquid (frequently-used) or lyophilized |
| Concentration | About 1mg/ml. There are differences between batches. Special concentration could be customized. |
| Sterility | 0.2 μM filtered |
| Buffer | See the Datasheet |
| Storage | See the Datasheet |
Target
| Target | Cockroach Bla g 2 |
| Alternative Names | Cockroach Allergen Bla g 2; Bla g 2 |
| Introduction | Among such allergens, Bla g 2 is of particular importance, eliciting IgE responses in 58-70% of cockroach allergic patients. The X-ray crystal structure of Bla g 2 shows a bilobal fold typical of pepsin-like aspartic proteases, but Bla g 2 is enzymatically inactive due to amino acid substitutions in the catalytic site. The structures of both lobes are similar despite the low degree of the amino acid sequence homology (< 15% identity). The presence of a zinc binding site and five disulfide bridges in Bla g 2 adds stability to the protein, thus favoring persistence of the allergen in the environment. Chronic exposure to low doses (< 1 µg/g dust) of this stable cockroach allergen may explain the association between sensitization to Bla g 2 and asthma. |
| Organism | Parasite |
| Related Disease | Allergy |
| Infections Route | Air |
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