Key Details
| Catalog | MAS-0524-YJ9 |
| Applications | ELISA; WB |
| Host | Mouse |
| Reactivity | EHEC |
| Clonality | Monoclonal |
| Conjugations | Conjugation could be customized |
| Sub CAT. | Applications | Clone | Conjugations | Endotoxin Level | Size | Quantity | |
|---|---|---|---|---|---|---|---|
| MAS-0524-YJ9-A | ELISA, WB | Q1D5 |
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Overview
Properties
Target
Overview
| Description | Clone Q1D5 is a mouse monoclonal IgG recognizes EHEC Stx2A. |
| Reactivity | EHEC |
| Applications | ELISA; WB |
| Host | Mouse |
| Immunogen | Stx2A-GST |
| Isotype | Mouse IgG2a |
| Clonality | Monoclonal |
| Clone | Q1D5 |
| Dilutions |
ELISA: 0.1-1000 ng/mL WB: 0.2-1 μg/mL |
Properties
| Expression Host | HEK293F/CHO |
| Conjugations | Conjugation could be customized |
| Purification | Protein A/G affinity purified is the regular method |
| Purity | SDS-PAGE> 95% |
| Endotoxin Level | Endotoxin level could be customized |
| Form | Liquid (frequently-used) or lyophilized |
| Concentration | About 1mg/ml. There are differences between batches. Special concentration could be customized. |
| Sterility | 0.2 μM filtered |
| Buffer | See the Datasheet |
| Storage | See the Datasheet |
Target
| Target | EHEC Stx2A |
| Alternative Names | Enterohemorrhagic Escherichia coli (EHEC); Shiga toxin Type II A subunit; EHEC; Enterohemorrhagic Escherichia coli; Stx2A |
| Introduction | Enterohemorrhagic Escherichia coli (EHEC) consists of multiple serotypes, among which O157:H7 is the one most commonly linked to epidemic and sporadic disease in humans throughout Japan, North America, and parts of Europe. EHEC O157:H7 infections are a primary cause of hemorrhagic colitis and the hemolytic-uremic syndrome. The pathogenesis of EHEC infections is associated with the production of Shiga toxins that are similar to the Shiga toxin that is produced by Shigella dysenteriae type 1. EHEC produces two Shiga toxins, Stx1 and Stx2, and ∼56% of their nucleotide sequences are identical. Both stx1 and stx2 are found within the late operons of Stx-encoding phages. Stx2 production is greatly induced by mitomycin C, suggesting that stx2 can transcribe from the late phage promoter of the Stx2-encoding phage. |
| Organism | Bacteria |
| Related Disease | Haemolytic-uraemic syndrome |
| Infections Route | Alimentary canal |
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