Seaweed Bromoform Content Analysis Service

Reliable Bromoform Analysis Service in Seaweed at Creative Biolabs

Seaweed absorbs carbon dioxide, water, and sunlight to grow through photosynthesis, and also absorbs inorganic substances in seawater, including bromide. During the biological metabolism of seaweed, part of the bromide may be converted into organic halides, such as bromoform. This organohalogenide is usually a volatile organic compound that can accumulate in seaweed and eventually be released into the surrounding environment, and may also be passed to other marine organisms through the food chain. Bromoform is highly toxic and affects the central nervous system through inhalation and skin absorption. Due to the influence of the marine environment, harvested seaweed products may contain varying levels of bromoform. To better realize the commercialization of seaweeds, bromoform must be accurately quantified to ensure that they do not exceed safe limits. Moreover, analyzing the bromoform content in seaweed also helps monitor changes in the marine environment and pollution levels. Creative Biolabs has a reliable Seaweed Profiling platform, and our scientists provide clients with professional seaweed bromoform content analysis service through years of analysis experience.

• Sample preparation

For the detection of volatile bromoform, sample preparation is crucial. Seaweed is composed of different structures such as leaves, stems, and roots. All structures should be mixed during sampling and the moisture in fresh samples should be removed by freeze-drying. The dried seaweed samples are then crushed or cut to make them easier to mix and extract.

• Bromoform extraction and enrichment

Methanol, hexane, pentane, and methylene chloride are commonly chosen as solvents for extracting bromoform because they are all volatile and compatible with gas chromatography (GC). We place the prepared seaweed sample and solvent in an extraction bottle to fully contact the sample with the solvent and then perform shaking extraction. The mixed solution after shaking and extraction is then centrifuged with a centrifuge to separate the upper organic phase (containing the organic matter to be measured) and the lower aqueous phase. Finally, the organic solvent in the organic phase is removed to achieve the concentration of the components to be measured.

• Bromoform analysis

We analyze bromoform in seaweeds by GC or gas chromatography-tandem mass spectrometry (GC-MS). We introduce the sample into the GC system. Under the conditions of the inert gas mobile phase, each component in the sample will be separated in the chromatographic column according to its boiling point and polarity and retained to varying degrees. Finally, quantitative analysis of bromoform is achieved through different detectors. A suitable detector is very important for the quantification of analytes. We have a variety of detectors for bromoform analysis, such as mass spectrometry detector (MS), electron capture detector (ECD), and flame ionization detector (FID).

In addition, some non-chromatographic quantitative methods are also used for the analysis of bromoform, such as infrared spectroscopy (IR), fluorescence spectroscopy, nuclear magnetic resonance (NMR), colorimetry, etc.

Creative Biolabs has experienced technical teams that are proficient in operating analytical instruments, extracting and separating seaweed samples, and providing clients with high-quality, reliable seaweed bromoform content analysis services to meet your needs and win trust. In addition to bromoform analysis, we also provide Sugars, Element, Fatty Acid, and Phytohormones Analysis services in seaweed. Please contact us for details if you need to inquire about a service.

Published data

Bromoform (CHBr3), commonly found in the red seaweed Asparagopsis, can effectively reduce methane production by ruminants. After seaweed is harvested, since bromoform is volatile, its concentration will be affected by the processing and storage environment, so it needs to be accurately quantified. The authors developed a convenient and simple GC-MS method for the quantitative determination of bromoform content in biomass red seaweed and conducted a comprehensive and detailed method validation of the developed detection method, including sensitivity, precision, linearity, stability, and accuracy. The results showed that this method had been recognized by testing agencies and used to evaluate the content of bromoform in seaweed. Asparagopsis can be used as a feed additive to reduce methane emissions from livestock. The detection method developed by the authors therefore plays an important role in processing seaweed into potential feed additives.

Fig.1 Analysis of bromoform in the red seaweed.¹

FAQs

Q1: What range of bromoform content can be detected by the method you use?

A1: Our method can generally detect bromoform content in the low to high ppm range, but the specific range may depend on the sample pretreatment method and analysis conditions.

Q2: How are the fees for your analytics services calculated? Are there any quantity discounts?

A2: The cost of analysis depends on factors such as sample quantity and sample complexity. We usually offer quantity discounts, please contact us for a quote on specific costs.

Q3: Can your analytical method be adapted to different species of seaweed?

A3: Our analysis methods are generally applicable to various types of seaweed samples, but the specific situation may vary depending on the sample characteristics, and we will make adjustments according to the actual situation.

Q4: How do you deal with possible interfering substances in your sample?

A4: We usually use appropriate pre-treatment methods or select appropriate analysis conditions to reduce or eliminate interfering substances in the sample.

Customer Review

Reference

1. Romanazzi, Donato, et al. "Rapid analytical method for the quantification of bromoform in the red seaweeds Asparagopsis armata and Asparagopsis taxiformis using gas chromatography–mass spectrometry." ACS Agricultural Science & Technology 1.5 (2021): 436-442.

Related Services:

1. Seaweed Simple Sugar Quantitative Profiling
2. Amino Acid Composition Profiling
3. Aflatoxin Profiling
4. Seaweed Elemental Quantitative Analysis
5. Seaweed Multi-element Simultaneous Profiling
6. Fatty Acid Composition Analysis
7. Pigment Quantitative Analysis
8. Seaweed Plant Hormones Determination
9. Seaweed Vitamers Quantitative Analysis
10. Total Phenolics Analysis
11. Phenolic Composition Analysis
12. Seaweed Tannin Qualitative and Quantitative Profiling
13. Alginate Molecular Size Analysis
14. Seaweed Total Phlorotannins Content Analysis