Key Details
| Catalog | MAS-0524-YJ13 |
| Applications | ELISA |
| Host | Mouse |
| Reactivity | Brucella |
| Clonality | Monoclonal |
| Conjugations | Conjugation could be customized |
Overview
Properties
Target
Overview
| Description | Except for the mAb 6B3, all mAbs recognise the S-LPS of B. abortus W99 (coated at 0.3 μg/ml) with a relative reactivity ranked as follows: 4F9> 12B12> 2C8. At the same LPS concentration, the reactivity of the two anti-C epitope antibodies and the anti-M epitope antibodies on B. melitensis Rev1 LPS is ranked as follows: 12B12> 6B3> 12G12. No reactivity was observed with the anti-A mAb 4F9 and 2C8 at low concentration of B. melitensis Rev1 LPS (0.3 μg/ml). Unexpectedly, the mAb 4F9 is not the only presenting some reactivity with the LPS of Y. enterocolitica O:9 since the mAb 2C8 recognised it almost as well. The mAb 6B3 is unable recognise the B. abortus W99 LPS even at highest LPS concentration. The mAb 2C8 shows weak reactivity with the highest concentration of Rev1 LPS. |
| Reactivity | Brucella |
| Applications | ELISA |
| Host | Mouse |
| Epitope | O-chain |
| Isotype |
6B3, 2C8: Mouse IgG3 kappa 4F9: Mouse IgG2a kappa 12B12: IgG3 lambda |
| Clonality | Monoclonal |
| Clone | 6B3; 2C8; 4F9; 12B12 |
| Dilutions | ELISA: 0.1-1000 ng/mL |
Properties
| Expression Host | HEK293F/CHO |
| Conjugations | Conjugation could be customized |
| Purification | Protein A/G affinity purified is the regular method |
| Purity | SDS-PAGE> 95% |
| Endotoxin Level | Endotoxin level could be customized |
| Form | Liquid (frequently-used) or lyophilized |
| Concentration | About 1mg/ml. There are differences between batches. Special concentration could be customized. |
| Sterility | 0.2 μM filtered |
| Buffer | See the Datasheet |
| Storage | See the Datasheet |
Target
| Target | Brucella LPS |
| Alternative Names | Brucella; LPS; Lipopolysaccharide |
| Introduction | Brucellae are gram-negative facultative intracellular bacteria infecting humans and domestic animals. The O-chain of Brucella LPS bears two distinct antigens, designated A and M. The variable ratio of A and M antigen in smooth Brucella strains defined their A- and/or M-dominant character. |
| Organism | Bacteria |
| Related Disease | Brucellosis |
| Infections Route | Oral cavity, alimentary canal |
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