3D Human Blood-Brain Barrier Spheroid Model Introduction

Creative Biolabs offers a cutting-edge 3D human blood-brain barrier (BBB) spheroid model for in vitro drug screening and neuroscience research. Our BBB spheroid model is a physiologically relevant platform that mimics the in vivo environment of the human brain, providing more accurate and predictive data than traditional 2D models. Our team of experts can assist you to study drug permeability, toxicity, and efficacy across the BBB in a cost-effective and high-throughput manner.

Fig 1. Structural organization of the Neurovascular unit (NVU) and the Blood-Brain Barrier (BBB).¹

BBB Spheroid Models

To date, human immortalized cell-based multicellular spheroidal BBB models (hiMCS-BBB models) are widely used in BBB research. Human brain endothelial cells (ECs), pericytes, and astrocytes are essential for the BBB structure. The hiMCS-BBB consisting of human immortalized brain ECs, astrocytes, and brain pericytes are co-cultured under low-adherence conditions. Among the main types of BBB models, like transwell 2D models, hydrogel models, hollow fiber models, and microfluidic models, BBB spheroids stand out with their distinguished advantage of closely mimicking the real blood-brain barrier. This showcases their significance in providing advanced and innovative insights into the blood-brain barrier function.

Advantages

The spheroid model offers several advantages over other in vitro models, including:

• More physiologically relevant environment.
• Larger surface area for cell-cell interactions.
• Easier manipulation, and a more accurate representation of complex cell-cell interactions and structures.
• An average diameter ranging from 200 to 400 μm, makes them suitable for high-throughput applications since they can be analyzed for fluorescence studies as a whole mount without the need for prior cutting.

Applications of BBB Spheroid Models in Pre-Clinical Study

In vitro BBB models enable transport and visualization studies, drug transporter functionality analysis, drug interaction and targeting studies, pharmacological safety assurance, examination of BBB functions relevant to disease, and drug discovery research. Implementing the factors, such as dynamic shear stress, intercellular communication, and cellular polarity might be decisive in the future development of personalized and disease-relevant BBB models.

Creative Biolabs is a prominent contract research organization that offers a wide range of customized services and comprehensive solutions in 3D-based spheroid models. Apart from the Human Blood-Brain Barrier Spheroid Model, you may also be interested in:

Related Services

Our dedicated teams are well-equipped to cater to the diverse needs of our clients, from laboratories to industries, and provide tailored solutions to meet their specific requirements empowered by a series of 3D biology-based assay services. If you have any needs in 3D biology models, please do not hesitate to contact us.

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• Hu-RPE Spheroid
• HCO Spheroid
• HC-A Spheroid
• hCSs
• HSteEC Spheroid
• HSC Spheroid
• HSC Monoculture Spheroid
• HPAEpiC Spheroid
• Hu-Airway Spheroid
• Tumor Spheroid