3D Organoid Model

The organoid model is able to precisely simulate complex in vivo environments and offer a dynamic tool for both fundamental research and clinical transformation. Creative Biolabs is devoted to providing a broad series of customized 3D organoid models to help your project achieve great success.

Overview of 3D Organoid Model

Organoids are essentially 3D cultures obtained from multiple categories of cells, such as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), somatic stem cells (SCs), and tumor cells. Among them, the first three kinds of cells are cultured into normal organoids on an extracellular matrix with or without differentiation. By contrast, tumor cells will be induced to form tumoroids in well-defined 3D cultures. The preparation of different kinds of organs requires different cultural compositions. The additive formulations utilized will still keep diverse in elements even for two different tissues with similar appearances, for instance, the small intestine and colon.

Fig.1 Organoid establishment from stem cells and cancer cells. (Xu, 2018)

Advantages of 3D Organoid Models

3D organoid models can be constructed from multiple types of organs and show several incomparable advantages over 2D cell culture and classic animal in vivo models.

• These models are very close to primary tissues in either composition or structure, containing a small number of stem cells with self-renewing ability, whose progeny have cell lineages similar to the living tissue.
• It's easy to achieve the rapid amplification and cryopreservation of organoids; hence, they are suitable for application in high throughput analysis.
• Certain organoids lack interstitial cells, enabling the research of interested tissue types without local micro-environment interference.
• Compared with monolayer culture models, 3D organoid models are more physiologically relevant and easier to manipulate niche components and signaling pathways and undertake genome editing.
• Organoids have the advantages of a high success rate of construction and fast culture speed. The process, from sample collection to drug sensitivity results, can be well controlled within two weeks.
• The clinical relevance and predictive effectiveness of organoids for cancer drug screens have been fully confirmed in several studies.

Applications

As powerful technology bridges conventional 2D in vitro models and in vivo animal models, 3D organoid models promote the translation from fundamental research to clinical practice. Such models are playing an increasingly important role in the preclinical stage.

Fig.2 Applications of 3D organoid models. (Creative Biolabs)

Hot Organoid Models And Related Services at Creative Biolabs

Creative Biolabs has developed an extensive organoid models to accelerate your project development, which including but not limited to 3D Tumor Organoid Model, 3D Normal Organoid Model, and Bespoke 3D Organoid Model. Based on existing organoid models, we have launched a number of supporting services listed below to promote your drug discovery process. For more details, please feel free to contact us.

Related Sections:

• 3D Spheroid Model
• Organ-on-a-Chip Model Introduction
• Ex Vivo Tissue Model
• Precision-Cut Tissue Slicing Model