Pain-Related Functional Assays

As a common symptom and one of the main features of diseases, pain affects the quality of life of many patients. Therefore, it is important to develop drugs that target pain or to assay whether other drug candidates cause pain. Creative Biolabs provides customers with scientific basis and customized solutions through pain-related functional assays to accelerate the development of drug candidates.

Pain Mechanism Overview

Pain is a physiological or pathological response of the body to tissue damage or abnormal stimuli. Its mechanism involves the multi-step transmission of pain signals. In this process, neurons are the main players.

Pain perception: When tissue is damaged, inflammatory, or otherwise stimulated, specialized pain-sensing neurons are activated and generate electrical signals.
Pain signal transduction: At the synapse, these electrical signals are transmitted to the next neuron through the release of neurotransmitters.
Pain signal transmission: These electrical signals are transmitted to the central nervous system through nerve fibers.
Pain signal processing: In the central nervous system, these electrical signals continue through multiple brain regions and are modulated and integrated to generate the perception and response to pain.

First neuron (in blue) of the ascending pain pathway. (Petroianu, 2023) Fig.1 First neuron (in blue) of the ascending pain pathway.¹

Indispensable Pain-Related Functional Assays in Drug Development

In order to evaluate the therapeutic potential and safety of candidate drugs, pain-related functional assays must be performed to provide the scientific basis for drug development and accelerate the development of candidate drugs.

Understand the action mechanism of the drug	Evaluate pain relief effect or adverse reaction
Through pain-related functional assaying, the effect of candidate drugs on key links such as pain conduction and neurotransmitter release can be studied. This provides important guidance for drug development.	The pain relief effect or pain-inducing degree of a drug is one of the key indicators for drug development. Through pain-related functional assaying, the effects of candidate drugs on pain perception, conduction, and regulation of the central nervous system can be evaluated to determine the effects of drugs on pain.
Optimize drug design and dosage optimization	Support clinical trial design
Pain-related functional assaying can provide data on the impact of drugs on pain-related signaling pathways, helping to optimize drug design and dosage optimization. This helps to improve the specificity, efficacy, and safety of the drug and reduce adverse reactions and side effects.	Pain-related functional assay results can provide an important basis for the design of clinical trials. According to the results of pain-related functional assays, appropriate clinical endpoint indicators, drug dosage, and treatment plans can be determined to improve the effect and success rate of clinical trials.

Pain-Related Functional Assaying Services at Creative Biolabs

Requirement confirmation and project initiation
Sample preparation and delivery
3D cell or organoid model construction
Treatment of candidate drugs at different concentrations
Electrophysiological assays
- Assessing neuronal excitability and conduction.
- Studying the effects of drug candidates on neuronal excitability, action potentials, and synaptic transmission.
Calcium imaging analysis
- Providing dynamic changes data of intracellular Ca²⁺ concentration
- Studying the effects of candidate drugs on neuron activity, neurotransmitter release, and cell signal transduction.
Data analysis and result interpretation

With a highly specialized team and rich experience and expertise, Creative Biolabs has significant service advantages and value in providing pain-related functional assaying services. Our services are not only highly customizable but also can carry out customized experimental designs according to the needs of customers. In addition, we also have a fast and high-throughput experimental platform to ensure accurate and reliable pain-related functional assaying data for customers. We sincerely invite you to contact us to accelerate your research.

Reference

Petroianu, GA.; et al. Neuropathic pain: mechanisms and therapeutic strategies. Frontiers in Cell and Developmental Biology. 2023, 11:1072629.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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