3D Human Hepatic Stellate Cell Monoculture Spheroid Model Introduction

Hepatic fibrosis, primarily orchestrated by the hepatic stellate cell (HSC), is the excessive accumulation of extracellular matrix (ECM) proteins including collagen that occurs in most types of chronic liver diseases. With the state-of-the-art 3D human HSC monoculture spheroid model, Creative Biolabs is committed to offering full support for your research in the pathogenesis and diagnosis of liver fibrosis and discovery of new therapeutic interventions.

Human hepatic stellate cell spheroid. (Li, et al., 2022)

Limitations of Conventional Models for Hepatic Fibrosis

Although many therapeutic methods are effective in experimental models for hepatic fibrosis, there are currently no approved treatment modalities in human. The in vitro liver models are widely used for liver fibrosis research. However, in traditional 2D cell culture, HSCs are maintained in an activated state, which impedes the investigation on the role of HSC in liver diseases. Traditional animal models have been instrumental in the study of liver fibrosis, but the notable variations between animal models and human patients in terms of metabolism and the regulation of disease-related genes continues to hinder the progress of effective drug development.

Hepatic stellate cell spheroid aggregation. (Song, et al., 2019)

Why Choose 3D Human HSC Monoculture Spheroid Model

The latest antifibrotic treatments are designed to inhibit the accumulation of fibrogenic cells and/or preventing the deposition of ECM proteins. Creative Biolabs has pioneered the construction of 3D human HSC monoculture spheroid using human primary hepatic stellate cells, which replicate the hepatic microenvironment better than conventional 2D monolayer models. Our model has undergone thorough validation, demonstrating that the clustered HSC cell aggregates manifest a quiescent state and express characteristic proteins. This model proves to be effective for investigating HSC signaling pathways in the context liver diseases.

Key Features

Our self-assembling hepatic spheroids will serve as an invaluable tool in exploring hepatocyte activation and the pathophysiological states of liver diseases. Numerous validations have substantiated that our 3D models offer superior fidelity in replicating the spatial organization and in vivo conditions of the liver's physiological and pathological states. By leveraging these models, you can better predict drug responses and toxicity, screen potential candidate drugs, and reduce reliance on animal testing.

3D Human Hepatic Stellate Cell Monoculture Spheroid Model Introduction

Creative Biolabs’ unwavering pursuit of excellence has culminated in the development of 3D human HSC monoculture spheroid model, revolutionizing the way researchers approach cellular studies. With its remarkable fidelity to human tissue architecture, the human spheroid model enables a deeper understanding of complex cellular interactions and disease mechanisms. In addition to 3D human HSC monocultured spheroids, we also prepare a series of human cell-based spheroid models for you to meet any anticipated research needs.

Related Services

Expanding upon our extensive range of 3D spheroid models, we envision a productive collaborative journey, enriched by the utilization of our specialized technical services in 3D biology, as detailed below. Please do not hesitate to contact us and discuss how our expertise can complement your strengths.

References

Lee, H.J.; et al. In vitro modeling of liver fibrosis with 3D co-culture system using a novel human hepatic stellate cell line. Biotechnology Bioengineering. 2022, 10: 28333.
Song, L.; et al. Assembly of human stem cell-derived cortical spheroids and vascular spheroids to model 3-D brain-like tissues. Nature. 2019, 9: s41598.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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