Precision-Cut Lung Slice Model Introduction

Creative Biolabs delivers unparalleled and trustworthy precision-cut lung slice (PCLS) models for research purposes. We understand the crucial role that precise and reliable models play in the disease research sector, and we are committed to providing researchers with superior resources to advance their investigations.

Respiratory Diseases and PCLS

It is a well-established fact that respiratory diseases are among the most common causes of death, following cardiovascular disease, stroke, and cancer, according to data from the World Health Organization. Despite significant investments in research and development, the incidence rates of major respiratory diseases such as idiopathic pulmonary fibrosis, acute lung injury, chronic obstructive pulmonary disease (COPD), and asthma have not shown any significant improvement over the past decade, or even increased in some cases.

Construction of PCLS. (Liu, 2019)Fig 1. Construction of PCLS. (Liu, 2019)

The lack of suitable translational models and the discrepancies between existing models and clinical phenotypes have limited the success of discovering new drug therapies in many disease fields. However, precision-cut tissue slices offer a solution to this challenge by providing a reliable way to evaluate efficacy and safety. These live explants with defined thickness and diameter, prepared from animal or human lung tissue and cultured ex vivo, are crucial in overcoming the challenges faced in drug discovery.

Why Choose Creative Biolabs' PCLS

Traditional Respiratory Disease Model

Animal models still hold great value for the study of respiratory diseases. However, data gathered from genetically similar animals living in controlled environments do not necessarily apply to human diseases. To address this issue, researchers are exploring in vitro human systems such as air-liquid interface (ALI) cultures and organ-on-a-chip models. However, despite being essentially 3D, these models struggle to recreate the intricate cellular interactions and complex structural and cellular arrangements of the airways and parenchyma in the lung, making it difficult to simulate the complexity of cellular interactions in the lung.

Precision-Cut Lung Slice Model

PCLS is an invaluable tool for researching respiratory diseases due to its ability to retain the lung's structure. Morphologically, PCLS preserves important tissue architecture, including small airways, respiratory parenchyma, structural and immune cell populations, and connective tissue. With regards to cellular composition, PCLS maintains most of the structural and immune cell populations of regions of variation or distinctive features, particularly in cases where disease related changes are distributed heterogeneously. It provides a unique opportunity to correlate cell-specific functions with organ physiology. PCLS can be obtained from naïve, transgenic, or diseased animals, as well as human surgical samples and lung explants.

Key Features

Creative Biolabs offers PCLS of exceptional quality, which enables researchers to obtain an accurate portrayal of in vivo conditions, thereby reducing the requirement for animal experimentation and expediting the drug development process.

High-Precision Cutting

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PCLS prepared in reproducible and uniform slices with a thickness of 100-500 μm.

Morphological Preservation

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Technical challenges are overcome to preserve the honeycomb-like structure and tight connections between extracellular matrix and cells.

Cell Viability Preservation

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The sample cells are with extremely high viability to avoid any loss of genes or proteins.

Multiple Applications

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Can be used for evaluating asthma, COPD, idiopathic pulmonary fibrosis, allergies, infections, and toxicological research.

Our Services

Our company provides the most advanced and reliable predictive PCLS models for cellular toxicity testing. With our cutting-edge PCLS technology, we are at the forefront of translational research. Particularly, our PCLS can be obtained from a variety of animals, including those that are naive, transgenic, or diseased, as well as human surgical samples and lung transplants. These samples can be cryopreserved for future use. Contact us now to obtain the best model that combines the complexity and functionality of any tissue.

References

  1. Liu, G.; et al. Use of precision-cut lung slices as a translational model for the study of lung biology. Respiratory Research. 2019, 20: 162.
  2. Hire, S.; et al. Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices. Nature. 2014, 5: 2440-2449.
  3. Ruigrok, M.; et al. siRNA-mediated protein knockdown in precision-cut lung slices. European Journal of Pharmaceutics and Biopharmaceutics. 2018, 133: 339-348.
  4. Li, G.; et al. Preserving airway smooth muscle contraction in precision-cut lung slices. Nature. 2020, 10: 6480.
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