3D Biology Based Target Analysis and Validation Services

The identification, management, and monitoring of many diseases rely heavily on biomarkers. The patient's disease condition, treatment response, and disease progression can all be swiftly and precisely assessed by looking for certain biomarkers. High-throughput sequencing can aid in the initial identification of prospective biomarkers; target analysis and verification are then necessary to further filter and verify putative biomarkers. The three-dimensional (3D) biology-based target analysis and verification services provided by Creative Biolabs can better simulate the physiological environment of human tissues and provide more accurate biological data for the discovery of biomarkers.

Why Need Target Analysis and Validation?

Candidate biomarkers can be screened more finely through in-depth target profiling, helping the selection of biomarkers that are closely related to molecular mechanisms of specific diseases.
Through in-depth target validation, a comprehensive assessment of the specificity, sensitivity, stability, and reproducibility of a candidate biomarker can be performed to determine the authenticity and credibility of the biomarker.

Advantages of 3D Biology Based Target Analysis and Validation

Organoids are 3D cell clusters that are self-assembled in vitro by pluripotent stem cells or organ-specific progenitor cells, simulating the structure and function of corresponding organs in vivo. At present, the 3D biological research technology based on organoids has been widely used in the research of simulating human diseases and has rapidly become a research hotspot.

Fig.1 Advanced screening technologies.¹

In the study of biomarker discovery, target analysis and verification based on 3D biological research technology has the following advantages.

Advantage 1: Compared with a single cell culture model, the use of constructed organoids to process biomarkers can more accurately simulate complex physiological systems in vivo, and better reflect the expression changes of biomarkers and accumulation of upstream and downstream molecules in different types of cells in the same tissue or organ.
Advantage 2: Compared with comparable animal models, organoid-based target analysis and validation is a bridge connecting animal research and human clinical trials, which can improve research efficiency, greatly reduce trial-and-error processes and research costs, and shorten the biomarker discovery cycle. This helps to quickly launch new markers that may be used in clinical practice.

Featured Target Analysis and Validation Services

Gene Editing Organoid Based Target Analysis and Validation Services

Gene-edited organoids, which have been specifically modified to transform into specific gene defects, gene mutations, or gene overexpression organoids, can more accurately assess the role of biomarkers. Creative Biolabs specially launched gene editing organoid-based target analysis and validation service to help customers explore the role of candidate biomarkers in key processes, such as cell proliferation, migration, and differentiation from forward or reverse, as well as the role of candidate biomarkers in specific disease development.

Off-Target Screening Cell Microarray Assay

Although the target analysis of biomarkers helps further determination, the binding or interference of non-target proteins may have a certain impact on subsequent research and even lead to the failure of the entire research. To avoid this from happening, off-target analysis experiments are required. To help customers discover and solve off-target problems in advance, Creative Biolabs specially launched the off-target screening cell microarray assay service to help large-scale screening of the interaction between candidate biomarkers and molecules other than target molecules in cells.

Creative Biolabs is a biotechnology company with many years of experience, focusing on providing the world's most advanced 3D biology-based biomarker discovery and development services. With rich experience, advanced equipment, and customized services, we can provide you with the most comprehensive 3D biology-based target analysis and verification services. If you need on-target analysis and off-target analysis in the process of searching for potential biomarkers, please contact us.

Reference

Booij, TH.; et al. Tumor organoids as a research tool: how to exploit them. Cells. 2022, 11(21).

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

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