Brain Endothelial Cell Monoculture Model for Neurological Disorder Drug Discovery and Development

Creative Biolabs presents a monoculture model of brain endothelial cells, furnishing an intrinsically refined platform to propel pharmaceutical innovation in the realm of drug investigation and development on behalf of our clientele.

Brain Endothelial Cells Overview

The blood-brain barrier (BBB) is composed of specialized microvascular endothelial cells, along with pericytes, astrocytes, microglia, neurons, and a basement membrane. The capillary endothelial cells are interconnected through proteins such as occludin, claudins, and junctional adhesion molecules, which form tight junctions (TJs). These TJs effectively seal the intercellular gaps, thereby limiting the permeability of central nervous system (CNS)-active substances.

Fig.1 Schematic depiction of the blood-brain barrier permeability for different drug-like chemical substances.¹

More than 98% of existing therapeutic agents face challenges in traversing the blood-brain barrier (BBB) as a result of inherent molecular attributes and physicochemical considerations, encompassing characteristics such as hydrophilicity, hydrophobicity, polar surface area, molecular dimensions, and charge. Furthermore, BBB dysfunction significantly underlies a spectrum of conditions, including autoimmune and neuropathological processes such as Alzheimer's and Parkinson's diseases, neuroinfections like meningitis and encephalitis, as well as hemorrhagic and ischemic stroke, along with traumatic brain injury.

Approaches of Brain Endothelial Cells Monoculture Model

Transwell Systems

Utilize Transwell systems, where brain endothelial cells are cultured on porous membranes. This enables the study of drug permeability and barrier function.

Barrier Integrity Assays

Employ assays like TEER measurements and fluorescent markers to assess barrier integrity and evaluate drug penetration.

3D and Organ-on-Chip Models

Develop three-dimensional cultures or microfluidic organ-on-chip systems to better replicate the physiological complexity of the blood-brain barrier.

Creative Biolabs provides a monoculture model of brain endothelial cells, offering an in vitro experimental platform for the advancement of drug discovery and development targeting neurological disorders. Our establishment houses a seasoned cadre of scientific experts, distinguished by their profound acumen in the domain of cellular cultivation and tissue manipulation techniques. Please contact us for more information.

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