3D Biology Based Dendritic Cells Activation Assay

Dendritic Cells (DCs) Activation Assay

Mature DCs, exhibiting immune activation capabilities, effectively activate naïve T cells and serve as a central mediator in the initiation, regulation, and maintenance of immune responses. At Creative Biolabs, we unequivocally offer 3D biology-based DC activation assay services for a deeper understanding of immune system functionality.

DCs 3D co-culture system. Fig 1. DCs 3D co-culture system.1

Streamlined Procedure

Creative Biolabs aims to tailor each experiment according to your needs and scientific objectives, from standardized DC activation stimuli and conditions to specific maturation factors, immunomodulators, and detection parameters.

  • Stimulate DCs with various stimuli, ranging from pathogen-associated molecular patterns (PAMPs) to specific antigens or danger signals.
  • Employ flow cytometry or imaging techniques to assess changes in dendritic cell surface markers, enabling the characterization of their activation phenotype.
  • Utilize multiplexed immunoassays to measure the secretion levels of cytokines and chemokines, providing valuable information about dendritic cell function.
  • Our advanced analysis software facilitates the interpretation of experimental results, allowing for a comprehensive understanding of dendritic cell activation and maturation.

Value Proposition of 3D-based Biology

By selecting our exceptional and comprehensive services, you are choosing:

Biological Models:

Whether it's gels, 3D co-culture models, cell spheroids, tumor tissues, or microfluidic devices, we assist in selecting the appropriate model based on your specific scientific inquiries.

Cutting-edge Technology:

We employ advanced techniques to ensure accurate and precise measurements of DC activation markers and cytokine secretion levels.

Comprehensive Analysis:

Our assay provides a comprehensive view of DC activation by simultaneously evaluating multiple phenotypic markers, cytokines, and chemokines.

Customizable Approach:

Tailor the assay to your specific research goals by selecting stimuli, maturation factors, or immune modulators that align with your experimental requirements.

Tailored Services for Your Needs

Creative Biolabs is committed to providing the most reliable 3D biology-based assays with high sensitivity, specificity, and accuracy. With years of experience, we understand that successful research requires more than a singular focus. That's why we have developed a comprehensive service portfolio encompassing various aspects of biological testing, analysis, and consulting. Contact our dedicated team today to discuss your specific requirements regarding Creative Biolabs’ 3D biology-based assay services.

Reference

  1. Mizoguchi, Mio, et al. " Prediction of chemical respiratory and contact sensitizers by OX40l expression in Dendritic cells Using a novel 3D coculture system. " Frontiers in Immunology. 33 (2017): 0929.
Research Model

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