3D Biology Based Fibroblast Activation Assay

As key participants in the wound healing response, fibroblasts become activated in response to tissue injury. Creative Biolabs' commitment to assessing fibroblast activation provides essential data support to gain a deeper understanding of cellular responses and evaluate drug efficacy.

Traditional Fibroblast Activation Assay

Fibroblasts contribute significantly to wound healing, tissue remodeling, extracellular matrix (ECM) deposition, and fibrotic diseases. Fibroblasts exhibit vigorous proliferation when cultured on rigid 2D surfaces and form broad lamellipodia and stress fibers. However, this simplified culture method is considered inadequate for reproducing the physiological conditions of connective tissue cells.

3D Biology-Based Fibroblast Activation Assay

Creative Biolabs offers reliable fibroblast activation detection services through our 3D culture systems that are considered an advanced alternative for studying fibroblast behavior with the incorporation of a wide range of biological features and interactions.

Biomimetic Models:

Our 3D culture systems faithfully mimic the environment of fibroblasts, accurately representing both quiescent and activated phenotypes of the cells.

High Sensitivity:

Our optimized detection approaches allow us to detect subtle changes in fibroblast activation at even the lowest levels, ensuring high sensitivity in capturing the nuances of cellular responses.

Fig 1. Fibroblasts cultured in different states. ¹

Our Expertise and Offerings

We provide scalable and physiologically relevant fibroblast activation detection services to scientists worldwide, using a combination of structural and chemical composition reconstruction techniques.

• Freely customize our systems based on your specific requirements, including chemical cues, microstructural features, mechanical properties, and integrin binding sites.
• Simulation of various types of fibrosis by customized culture protocols, allows us to track changes in cytokines, protein composition, and matrix mechanics.
• We identify the activation state of fibroblasts through increased expression of α-smooth muscle actin (αSMA), nuclear localization of yes-associated protein (YAP), and gene expression alterations.

In addition to fibroblast activation assay and related services, Creative Biolabs is committed to reshaping existing biological research through our cutting-edge 3D-based biology technology.

Our team of experts is equipped with the knowledge and expertise to support your research needs and offer tailored solutions that deliver reliable and actionable results. Contact us to experience the power of our comprehensive service ecosystem.

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services