Creative Biolabs has assembled experts in the application of 3D biological models to provide effective 3D biology-based immune checkpoint assays.
The target biological material for the construction of the 3D ex vivo model platform to be assayed needs to be first collected and isolated, e.g. from in vivo, ex vivo, stem cells, or tumor cells. After selecting the scaffold material for model growth support, the model is constructed and guided with bioactive factors in the normal or tumor state 3D model.
Specific antibodies are used to label immune checkpoints, including but not limited to bioassays and biochemical assays for PD-1, CTLA-4, CD47, OX40, and LAG3, as well as blockade bioassays for CD278. Notably, the constructed tumorigenic 3D model can naturally carry a state of simulated immune checkpoint blockade.
The labeled antibodies are combined with immune cells or tissues in the sample and the markers are visualized by methods such as fluorescent staining and histochemistry. Images are acquired and quantitative analysis is performed.
Fig. 1 Imaging of 3D tumor ex vivo models.1
Fig. 2 Screening the efficacy of CPI at individualized specific levels.2
Creative Biolabs has focused on expanding the application of 3D biological modeling for many years and involved technologies, such as multilayer stacking of cells and construction of internal vascular networks, to provide emerging 3D biology-based immune checkpoint assay services. For further information on how we may assist with your desired assay, please kindly contact us.
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