Leukemic Colony-Forming Cell Assays

Creative Biolabs supports leukemic colony-forming cell (L-CFC) assays to assist clients in studying the regulation of various hematological diseases and test responses to chemotherapeutic agents.

Leukemic Colony-Forming Cell (L-CFC)

L-CFC, which is considered as the clonogenic cell of lymphoblastic leukaemias, may act as progenitor cells to maintain the leukemic cell population in vivo. L-CFCs have several properties in common with normal myeloid progenitor cells, including self-renewal and a high thymidine suicide index. Colony expansion of L-CFC from the majority of patients needs exogenous colony-stimulating factors (CSF), which are often added via conditioning media with activated T cells, placental tissue, or media produced from different tumor cell lines.

Fig.1 Appearance of lymphoid colony on an agar plate.¹

General Procedure of L-CFC Assays

Importance and Applications of L-CFC Assays

L-CFC assays are indispensable tools that contribute significantly to advancing our understanding of hematopoiesis, stem cell biology, and their applications in drug development. L-CFC assays also allow the assessment of the effects and toxicology of various compounds on hematopoietic stem and progenitor cells. Additionally, understanding the behavior of hematopoietic stem cells through L-CFC assays is crucial for the development of stem cell-based therapies.

• Contribute to the understanding of CLL cell proliferation, leukemia pathogenesis, and intervention.
• Determine in vitro drug sensitivities and unique dietary needs, which could allow for the customization of personalized treatment.
• Measure the functional suppression of the growth of cancer progenitor cells and assess the efficacy of potential biotherapeutics in preventing the spread of cancer stem and progenitor cells.
• Find leftover clonogenic leukemic cells in peripheral blood cell suspensions and marrow.

Our Services

With advanced technology and equipment, Creative Biolabs is proud to provide reliable mesenchymal assay services designed to optimize and simplify your preclinical drug development research. Our unwavering dedication, combined with a strong foundation in the field of 3D biology research, makes us a reliable partner to support your research initiatives. Creative Biolabs delivers state-of-the-art technologies and top-notch 3D models, precisely crafted to meet your specific requirements. Our array of models includes, but is not limited to:

• 3D Spheroid Model
• Organ-on-a-chip Model
• 3D Ex Vivo Human Tissue Model
• Precision-Cut Tissue Slice Model

If you have inquiries regarding L-CFC assays or 3D models, please do not hesitate to contact us at any time.

Related Sections:

• CFC (Colony-Forming Cell) Assays
• High-content Imaging and Analysis
• Tissue Cross-Reactivity Studies
• Gastrointestinal Motility Assays
• Pain-Related Functional Assays