Lower Esophageal Sphincter Motility

Creative Biolabs presents lower esophageal sphincter motility assay services, streamlining the comprehensive assessment and identification of anomalous patterns and physiological mechanisms within the LES. Through these cutting-edge services, a diverse array of information can be acquired to help the drug development process.

Introduction of Lower Esophageal Sphincter

Lower esophageal sphincter (LES), located at the distal end of the esophagus, plays a crucial role as a "live valve" to prevent the backflow of gastric contents into the esophagus.

Fig.1 Structural characteristics of the LES.¹

Approaches of LES Motility Assay

Esophagram	Upper Endoscopy
The esophagram represents a radiographic exploration of the esophagus, entailing the patient's ingestion of barium (a contrast agent) and subsequent visualization of the esophagus and stomach using fluoroscopy techniques. Application: Examine aids in the identification of potential esophageal strictures; Provide an approximate assessment of the intensity of esophageal muscle contractions.	Upper endoscopy is a diagnostic procedure enabling direct visualization of the esophageal, stomach, and upper small intestine (duodenum) linings using an endoscope passed through the oral cavity. Application: Evaluate and assess potential damage, such as esophagitis and ulcers.
Esophageal Manometry	Ambulatory 24-hour pH Probe
Esophageal manometry entails the insertion of a narrow tube through the nasal passage into the esophagus. The measurement of esophageal function is achieved by recording the pressure readings of esophageal muscle motility. Application: Assess the LES muscle pressure; Interpret potential issues with esophageal motility or the functioning of the LES; Determine dysphagia.	The procedure involves the insertion of a slender tube through the nasal passage into the esophagus, positioned at the level of the LES. Equipped with a pH sensor at its tip, this tube enables the collection of acid exposure measurements within the esophagus, which are recorded on a portable computer. The pH probe is worn continuously for a duration of 24 hours. Application: Be gold standard to determine reflux disease or GERD.

LES Motility Disorders

The LES functions as the paramount component of the anti-reflux barrier at the esophagogastric junction. Abnormal LES pressure can lead to various motility disorders.

Gastro Esophageal Reflux Disease (GERD)

GERD is a prominent disorder of the upper gastrointestinal tract, is characterized by acid reflux, heartburn, intractable cough, and asthma resulting from the regurgitation of upper gastrointestinal contents into the esophagus.

Dysphagia

Characterized by impaired peristaltic motility (with diminished propulsive contractions), the LES fails to fully relax, resulting in food

passage obstruction and esophageal dilation.

Achalasia

Achalasia manifests as a progressive development of dysphagia and regurgitation whose neurogenic etiology is characterized by three aberrant esophageal motility findings—absence of peristalsis, elevated resting pressure of the LES, and compromised receptive relaxation of the LES.

Fig.2 Disruption of the anti-reflux barrier at the esophageal junction.¹

Other Gastrointestinal Motility Assays

Creative Biolabs has meticulously curated a comprehensive repertoire of platforms aimed at assessing the effects of drugs on the LES. Our platform embraces a diverse spectrum of LES motility assay approaches and a wide range of 3D biology-based models, rendering invaluable support to our esteemed clientele. Our high-quality models encompass a wide range, including but not limited to:

If you have any related inquiries, please feel free to contact us for further information.

Reference

Zheng, Zhi, et al. "Current advancement on the dynamic mechanism of gastroesophageal reflux disease." International Journal of Biological Sciences 17.15 (2021): 4154.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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