3D Biology Based Neutrophils Chemotaxis Assay

The robust immune response necessitates complex coordination of multiple cell types in both temporal and spatial dimensions to effectively respond to damage and infection, and establish self-tolerance. Creative Biolabs utilizes the principles and cutting-edge technology of 3D biology to investigate neutrophil migration, and unravel the enigma surrounding this crucial physiological process.

Neutrophils Chemotaxis and Immune Response

The quantification of neutrophil migration plays a pivotal role in elucidating the migratory behavior of neutrophils, promoting a deeper understanding of immune responses, inflammation, and the development of targeted therapies. 2D assays have been proven to be a convenient tool for studying the response of cells to natural or pharmacological modulators. However, cellular chemotaxis predominantly occurs in complex 3D environments in vivo.

Fig 1. Cell chemotaxis analysis.¹

Highlighted Characteristics of 3D Neutrophils Chemotaxis Assay

• Specialized 3D culture system: We have created an environment that closely mimics the extracellular matrix, offering a more physiologically relevant backdrop for investigating neutrophil migration.
• Well-defined gradients: Our optimized experimental setup allows for the creation of stable and controllable gradients of cytokines/compounds within the culture system.
• Dynamic cell imaging: We are able to provide dynamic, time-resolved data and precise quantification and tracking of individual cells. This facilitates obtaining detailed and accurate research results.
• Stringent quality control: We ensure that each detection is performed with strict accuracy, temporal precision, and high reproducibility. You can rely on our product to deliver reliable experimental data.
• Expert assistance: Our team of biological experts possesses years of research experience and will be with you throughout your study, addressing queries, answering questions, and helping with the analysis of detection data.

Operational Protocol

Fig 2. Workflow. (Creative Biolabs)

Unleashing Our Solutions

Creative Biolabs supports customizable experimental setups, including the selection of chemotactic factors, concentration gradients, downstream detection assays, and even 3D gradient generation through professional microfluidic devices.

If you have any further questions or require additional information about our services, please do not hesitate to contact us.

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services