Normal Intestinal Organoid Model

Creative Biolabs has made a lot of fruitful efforts in developing 3D organoids, such as high-quality and customizable normal intestinal organoid (NIO) models. We will continue to improve our technical ability and expect to help your preclinical drug discovery projects achieve rapid progress.

Construction of Normal Intestinal Organoid Models

The construction methods of NIOs can be roughly divided into two kinds, which is just like Fair et al describe. One is generating organoids from intestinal biopsy or tissue from surgical resection, and the other starts with skin fibroblasts. In whatever way, NIOs are cultured in a specified 3D medium, although the ingredients of the medium vary greatly.

Fig.1 Schematic of human intestinal organoid creation. (Fair, 2018)

Advantages of Normal Intestinal Organoid Models

As a 3D ex vivo model, normal organoid models play an increasingly important role in research on various intestinal diseases owing to the following advantages.

• The emergence of organoids brings hope to the long-term in vitro culture of human intestinal epithelial cells. Such models can stably retain genetic integrity or tissue physiology.
• They reproduce key features of in vivo intestine, such as the villus and crypt domains, helping understand tissue/organ development, homeostasis, and diseases.
• In NIOs, almost all hallmarks of the small intestinal epithelium can be found, not only in the architecture but also in cell type composition and self-renewal dynamics.
• These models can reflect similar responses to the in vivo tissues and can be used to test drug toxicity.
• Owing to their expression of cystic fibrosis transmembrane conductance regulator (CFTR), NIOs offer a suitable tool for studies of cystic fibrosis.

Applications of Normal Intestinal Organoid Models

NIOs, as a novel 3D cell culture technology, can model a lot of intestinal disorders, further deepening our understanding of tumorigenesis and progression in studying intestinal cancers. Besides, they can be applied in antimicrobial drug screening, personalized medicine, virulence mechanisms, and pathogen-host interactions. In terms of toxicity testing, they facilitate the assessment of safety profiles of potential medicines before clinical testing. Furthermore, these models create a good platform for studying stem cell biology, and they are promising to generate transplantable epithelium for regenerative medicine.

Related Services

At Creative Biolabs, we have built a 3D organoid model network containing a series of normal organoid models generated from diverse tissues and prepared abundant stock to ensure the rapid arrival speed of these products. Our team is committed to providing our global customers with reliable products and professional 3D biology-based and assay services. As long as you have any needs in intestinal disease research, please feel free to contact us to discuss your project. Our team is here to help you achieve rapid success.

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• 3D Normal Organoid Model
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