Electrophysiology Assays

With many years of research experience, Creative Biolabs offers professional service of electrophysiological assays for you to electrodiagnose and monitor interneuron communication and study the effects of other physiological or pharmacological stimuli.

What are Electrophysiological Assays?

Electrophysiology is a subfield of physiology, that focuses on the electrical characteristics of biological cells and tissues. In electrophysiological assays, electrophysiological instruments, electrodes, voltage clamp, and patch clamp techniques are used to record or measure the potential changes, conduction velocity, and ion channels of whole animals or isolated organ tissues, nerves, and cell ion channels, etc. The assay features that minute electrical signals from action, membrane, or synaptic potentials are amplified and high throughput recorded using cutting-edge technology.

Electrophysiological Assays for Neuroscience

Electrophysiology techniques are widely used across a diverse range of neuroscience and physiological applications; from understanding the behavior of single ion channels in a cell membrane, to whole-cell changes in the membrane potential of a cell, to larger-scale changes in field potential within the brain slices in vitro. Electrophysiology assays are crucial for studying the electrical activity of neurons and understanding neural circuits. They enable researchers in their studies of the mechanisms underpinning brain function, synaptic transmission, plasticity, and illnesses like epilepsy, Parkinson's disease, and Alzheimer's disease.

Fig.1 Common in vitro electrophysiological methods.¹

Procedure of Electrophysiological Assays

• Preparation of Samples: For cellular or tissue-based assays, samples need to be prepared and maintained in a physiological environment. This may involve isolating cells from tissues or culturing specific cell types under controlled conditions.
• Electrode Placement: Electrodes are carefully positioned to enable the recording of electrical signals from the sample. The type and placement of electrodes depend on the specific assay and the biological system under study.
• Connection to Amplifiers and Data Acquisition Systems: Amplifiers are used to boost the weak electrical signals obtained from the samples, and data acquisition systems digitize and record the signals for subsequent analysis.
• Stimulation Protocols (if applicable): In some assays, electrical or chemical stimuli may be applied to the sample to evoke specific responses.
• Recording and Analysis: Electrical signals are captured and analyzed with the help of specialist software and algorithms. Parameters such as amplitude, duration, frequency, and waveform characteristics are quantified.

Electrophysiological Assays Services Provided by Creative Biolabs

Creative Biolabs has an experienced and competent scientific team to deliver customized service solutions to both academic and industrial customers in the research field of electrophysiology. With specialized equipment and extensive analytical experience, our services can offer valuable insight for your research. Using electrophysiological assays, which are represented by the current clamp assay, you can delve into neuroelectrophysiology and neuronal diseases, in addition, you can also conduct research in cardiac research, pain electrophysiology, etc.

Creative Biolabs offers a full range of in vitro electrophysiology assays to assist you in completing various tests and research. Please contact us to inform us of your requirements.

With professional model building knowledge and an excellent service team, Creative Biolabs promises to provide high quality experimental modes that cover a variety of types at the cell, organ, and tissue levels to meet your specific needs. The models we can provide include but are not limited to:

• 3D Spheroid Model
• Organ-on-a-chip Model
• 3D Ex Vivo Human Tissue Model
• Precision-Cut Tissue Slice Model

Related Sections:

• CFC (Colony-Forming Cell) Assays
• High-content Imaging and Analysis
• Tissue Cross-Reactivity Studies
• Gastrointestinal Motility Assays
• Pain-Related Functional Assays